验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, U-251 cells |
| Positive IHC detected in | human testis tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
产品信息
22838-1-AP targets C7orf30 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | C7orf30 fusion protein Ag18286 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 7 open reading frame 30 |
| 别名 | C7orf30, MALSU1 |
| 计算分子量 | 234 aa, 26 kDa |
| 观测分子量 | 26-28 kDa |
| GenBank蛋白编号 | BC012331 |
| 基因名称 | C7orf30 |
| Gene ID (NCBI) | 115416 |
| RRID | AB_11182483 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96EH3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C7orf30, also known as MALSU1, is a member of the iojap protein family. It was predicted to enable ribosomal large subunit binding activity. It was Involved in negative regulation of mitochondrial translation and ribosomal large subunit biogenesis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C7orf30 antibody 22838-1-AP | Download protocol |
| IHC protocol for C7orf30 antibody 22838-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Structural basis of GTPase-mediated mitochondrial ribosome biogenesis and recycling. | ||
Cell Rep The Human Mitochondrial DEAD-Box Protein DDX28 Resides in RNA Granules and Functions in Mitoribosome Assembly. | ||
bioRxiv Tetracycline-dependent inhibition of mitoribosome protein elongation in mitochondrial disease mutant cells suppresses IRE1α to promote cell survival | ||
EMBO Rep Tetracyclines activate mitoribosome quality control and reduce ER stress to promote cell survival |
