验证数据展示
经过测试的应用
Positive WB detected in | NIH/3T3 cells |
Positive IP detected in | NIH/3T3 cells |
Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 2 publications below |
产品信息
22159-1-AP targets CCZ1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IP, IHC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse |
免疫原 | CCZ1 fusion protein Ag17804 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | chromosome 7 open reading frame 28A |
别名 | C7orf28A, C7orf28B, CCZ1, CGI 43, H_DJ1163J12.2, UPF0550 protein C7orf28 |
计算分子量 | 482 aa, 56 kDa |
观测分子量 | 50-56 kDa |
GenBank蛋白编号 | BC132749 |
基因名称 | CCZ1 |
Gene ID (NCBI) | 51622 |
RRID | AB_2879011 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P86790 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for CCZ1 antibody 22159-1-AP | Download protocol |
IHC protocol for CCZ1 antibody 22159-1-AP | Download protocol |
IP protocol for CCZ1 antibody 22159-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Neuroinflammation Autophagy protein NRBF2 attenuates endoplasmic reticulum stress-associated neuroinflammation and oxidative stress via promoting autophagosome maturation by interacting with Rab7 after SAH. | ||
Biomedicines CCZ1 Accelerates the Progression of Cervical Squamous Cell Carcinoma by Promoting MMP2/MMP17 Expression
| ||
Autophagy GORASP2 promotes phagophore closure and autophagosome maturation into autolysosomes |