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验证数据展示

  • IP experiment of Ramos using 20880-1-AP

    IP experiment of Ramos using 20880-1-AP

    IP result of anti-C7orf23 (IP:20880-1-AP, 4ug; Detection:20880-1-AP 1:800) with Ramos cells lysate 1680 ug.

  • IHC staining of human urothelial carcinoma using 20880-1-AP

    IHC staining of human urothelial carcinoma using 20880-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 20880-1-AP (TMEM243 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human urothelial carcinoma using 20880-1-AP

    IHC staining of human urothelial carcinoma using 20880-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 20880-1-AP (TMEM243 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMEM243 Polyclonal antibody

TMEM243 Polyclonal Antibody for IHC, IP, ELISA
Cat No. 20880-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IP, ELISA

别名

C7orf23, MM TRAG, Transmembrane Protein 243, Transmembrane protein C7orf23

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IP detected inRamos cells
Positive IHC detected inhuman urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20880-1-AP targets TMEM243 in IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IP, ELISA Application Description
经测试反应性 human
免疫原 TMEM243 fusion protein Ag14979 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 7 open reading frame 23
别名 C7orf23, MM TRAG, Transmembrane Protein 243, Transmembrane protein C7orf23
计算分子量 118 aa, 13 kDa
观测分子量16 kDa
GenBank蛋白编号BC002837
基因名称 C7orf23
Gene ID (NCBI) 79161
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BU79
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C7orf23, also known as TMEM243, encodes a membrane protein that may be associated with drug tolerance in cells. The C7orf23 gene is capable of generating at least 12 different splice variants, 10 of which are capable of encoding 7 different protein isoforms, some of which contain transmembrane structural domains. Recent studies have shown that C7orf23 is significantly up-regulated and highly sensitive in blood samples from PD patients, revealing its potential as a future biomarker for PD diagnosis.

实验方案

Product Specific Protocols
IHC protocol for TMEM243 antibody 20880-1-APDownload protocol
IP protocol for TMEM243 antibody 20880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols