C7orf10 Polyclonal antibody

C7orf10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21589-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

Dermal papilla-derived protein 13, DERP13, EC:2.8.3.13, ORF19, Succinate--hydroxymethylglutarate CoA-transferase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inmouse kidney tissue, mouse pancreas tissue, mouse skeletal muscle tissue, HepG2 cells, HEK-293 cells, rat kidney tissue
Positive IHC detected inhuman kidney tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

21589-1-AP targets C7orf10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag16159

Product name: Recombinant human C7orf10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 123-471 aa of BC098318

Sequence: VKIIKELAAVCDVFVENYVPGKLSAMGLGYEDIDEIAPHIIYCSITGYGQTGPISQRAGYDAVASAVSGLMHITGPENGDPVRPGVAMTDLATGLYAYGAIMAGLIQKYKTGKGLFIDCNLLSSQVACLSHIAANYLIGQKEAKRWGTAHGSIVPYQAFKTKDGYIVVGAGNNQQFATVCKILDLPELIDNSKYKTNHLRVHNRKELIKILSERFEEELTSKWLYLFEGSGVPYGPINNMKNVFAEPQNAVSGFQSLLHSLAHGPFLHLQGSARVLHNGLVMEMEHPTVGKISVPGPAVRYSKFKMSEARPPPLLGQHTTHILKEVLRYDDRAIGELLSAGVVDQHETH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 7 open reading frame 10
别名 Dermal papilla-derived protein 13, DERP13, EC:2.8.3.13, ORF19, Succinate--hydroxymethylglutarate CoA-transferase
计算分子量 471 aa, 51 kDa
观测分子量 48-55 kDa
GenBank蛋白编号BC098318
基因名称 C7orf10
Gene ID (NCBI) 79783
RRIDAB_10734435
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HAC7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C7orf10 is also named as SUGCT, DERP13 and belongs to the CaiB/BaiF CoA-transferase family. It may function as part of a multiunit enzyme complex (A), similar to the alpha-ketoglutarate dehydrogenase system, or as the second of two independent enzymes, the first of which decarboxylates alpha-ketoadipate to glutarate (B). This protein has 4 isoforms produced by alternative splicing with the molecular weight from 43 kDa to 51 kDa.

实验方案

Product Specific Protocols
WB protocol for C7orf10 antibody 21589-1-APDownload protocol
IHC protocol for C7orf10 antibody 21589-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Mol Life Sci

Knockout of the non-essential gene SUGCT creates diet-linked, age-related microbiome disbalance with a diabetes-like metabolic syndrome phenotype.

Authors - Joanna Niska-Blakie
mouseWB

bioRxiv

Odd-chain dicarboxylic acid feeding recapitulates the biochemical phenotype of glutaric aciduria type 1 in mice

Authors - Adam C Richert
humanWB

Front Aging Neurosci

A Y374X TDP43 truncation leads to an altered metabolic profile in amyotrophic lateral sclerosis fibroblasts driven by pyruvate and TCA cycle intermediate alterations

Authors - Scott P Allen
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