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验证数据展示

  • WB analysis of PC-13 using 24588-1-AP

    WB analysis of PC-13 using 24588-1-AP

    PC-13 cells were subjected to SDS PAGE followed by western blot with 24588-1-AP (C6orf204 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 24588-1-AP

    IHC staining of mouse testis using 24588-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24588-1-AP (CEP85L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

CEP85L Polyclonal antibody

CEP85L Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24588-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (2)

别名

C6orf204, Serologically defined breast cancer antigen NY-BR-15, NY-BR-15, NY BR 15, Centrosomal protein of 85 kDa-like

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-13 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:300-1:800
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24588-1-AP targets CEP85L in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF, CoIP
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 CEP85L fusion protein Ag20235 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 6 open reading frame 204
别名 C6orf204, Serologically defined breast cancer antigen NY-BR-15, NY-BR-15, NY BR 15, Centrosomal protein of 85 kDa-like
计算分子量 805 aa, 92 kDa
观测分子量 56 kDa
GenBank蛋白编号BC153054
基因名称 CEP85L
Gene ID (NCBI) 387119
RRIDAB_2819012
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5SZL2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP85L, also named as C60rf204, is a gene which encodes a protein that has identified as a breast cancer antigen. Nothing more is known of its function at this time. There're five transcript variants with MW 45 kDa, 55-56 kDa and 91-92 kDa.

实验方案

Product Specific Protocols
WB protocol for CEP85L antibody 24588-1-APDownload protocol
IHC protocol for CEP85L antibody 24588-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Neuron

Pathogenic Variants in CEP85L Cause Sporadic and Familial Posterior Predominant Lissencephaly.

Authors - Meng-Han Tsai
View Article
humanWB,IF,CoIP

Neuron

Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.

Authors - Andrew Kodani
  • KD Validated
View Article