验证数据展示
经过测试的应用
| Positive WB detected in | PC-13 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:300-1:800 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24588-1-AP targets CEP85L in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag20235 Product name: Recombinant human C6orf204 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 146-496 aa of BC153054 Sequence: DMKDFRPLRKWSSLSKLTAPDNCGQGGTVCREESRNGLEKIGKAKALTSQLRTIGPSCLHDSMEMLRLEDKEINKKRSSTLDCKYKFESCSKEDFRASSSTLRRQPVDMTYSALPESKPIMTSSEAFEPPKYLMLGQQAVGGVPIQPSVRTQMWLTEQLRTNPLEGRNTEDSYSLAPWQQQQIEDFRQGSETPMQVLTGSSRQSYSPGYQDFSKWESMLKIKEGLLRQKEIVIDRQKQQITHLHERIRDNELRAQHAMLGHYVNCEDSYVASLQPQYENTSLQTPFSEESVSHSQQGEFEQKLASTEKEVLQLNEFLKQRLSLFSEEKKKLEEKVGFSNKVELGQQHFLSI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 6 open reading frame 204 |
| 别名 | C6orf204, Serologically defined breast cancer antigen NY-BR-15, NY-BR-15, NY BR 15, Centrosomal protein of 85 kDa-like |
| 计算分子量 | 805 aa, 92 kDa |
| 观测分子量 | 56 kDa |
| GenBank蛋白编号 | BC153054 |
| 基因名称 | CEP85L |
| Gene ID (NCBI) | 387119 |
| RRID | AB_2819012 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5SZL2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEP85L, also named as C60rf204, is a gene which encodes a protein that has identified as a breast cancer antigen. Nothing more is known of its function at this time. There're five transcript variants with MW 45 kDa, 55-56 kDa and 91-92 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CEP85L antibody 24588-1-AP | Download protocol |
| IHC protocol for CEP85L antibody 24588-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuron Pathogenic Variants in CEP85L Cause Sporadic and Familial Posterior Predominant Lissencephaly. | ||
Neuron Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.
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