验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, Jurkat cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25596-1-AP targets C6orf108 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag22336 Product name: Recombinant human C6orf108 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-174 aa of BC011683 Sequence: MAAAMVPGRSESWERGEPGRPALYFCGSIRGGREDRTLYERIVSRLRRFGTVLTEHVAAAELGARGEEAAGGDRLIHEQDLEWLQQADVVVAEVTQPSLGVGYELGRAVAFNKRILCLFRPQSGRVLSAMIRGAADGSRFQVWDYEEGEVEALLDRYFEADPPGQVAASPDPTT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 6 open reading frame 108 |
| 别名 | 2'-deoxynucleoside 5'-phosphate N-hydrolase 1, c Myc responsive protein Rcl, C6orf108, DNPH1, RCL, RP3 330M21.3 |
| 计算分子量 | 174 aa, 19 kDa |
| 观测分子量 | 23 kDa |
| GenBank蛋白编号 | BC011683 |
| 基因名称 | C6orf108 |
| Gene ID (NCBI) | 10591 |
| RRID | AB_2880150 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43598 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C6orf108 also named as DNPH1, catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5'-monophosphates to yield deoxyribose 5-phosphate and a purine or pyrimidine base. Deoxyribonucleoside 5'-monophosphates containing purine bases are preferred to those containing pyrimidine bases (PMID: 24260472). Expressed at low levels in brain, colon, lung, peripheral blood leukocytes, placenta, small intestine, and thymus. Expressed at high levels in heart, kidney, liver, skeletal muscle and spleen. Overexpressed in a significant proportion of breast cancers (PMID: 18726892).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C6orf108 antibody 25596-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
