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C5orf4 Polyclonal antibody

C5orf4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 22046-1-AP

Print datasheet

Synonyms

C5orf4, fatty acid hydroxylase domain containing 2, FAXDC2, Uncharacterized protein C5orf4

验证数据展示

  • IHC staining of human liver using 22046-1-AP

    IHC staining of human liver using 22046-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22046-1-AP (C5orf4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of COLO 320 using 22046-1-AP

    WB analysis of COLO 320 using 22046-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 22046-1-AP (C5orf4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inCOLO 320 cells, A549 cells, HepG2 cells
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

The immunogen of 22046-1-AP is C5orf4 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen C5orf4 fusion protein Ag16960 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 5 open reading frame 4
Synonyms C5orf4, fatty acid hydroxylase domain containing 2, FAXDC2, Uncharacterized protein C5orf4
Calculated Molecular Weight 333 aa, 39 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC007216
Gene Symbol C5orf4
Gene ID (NCBI) 10826
RRIDAB_2878979
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96IV6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fatty acid hydroxylase domain containing 2 (FAXDC2), also known as C5orf4, is a 40 kDa protein. The function of FAXDC2 remains largely unknown. The MW of this protein is 25 kDa, and this antibody specially recognises the 25 kDa protein.

实验方案

Product Specific Protocols
WB protocol for C5orf4 antibody 22046-1-APDownload protocol
IHC protocol for C5orf4 antibody 22046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Blood Cancer J

Novel function of FAXDC2 in megakaryopoiesis.

Authors - Q Jin
View Article
mouseWB

J Clin Invest

The cholesterol biosynthesis enzyme FAXDC2 couples Wnt/β-catenin to RTK/MAPK signaling

Authors - Babita Madan
View Article