验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33453-1-AP targets C4orf32 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag38764 Product name: Recombinant human C4orf32 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-99 aa of BC022534 Sequence: MCSAGELLRGGDGGERDEDGDALAEREAAGTEWDPGASQRRRGQRQKESEQDVEDSQNHTGEPVGDDYKKMGTLFGELNKNLINMGFTRMYFGERIVEP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 4 open reading frame 32 |
| 别名 | FAM241A, Family With Sequence Similarity 241 Member A, Uncharacterized protein FAM241A |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | BC022534 |
| 基因名称 | C4orf32 |
| Gene ID (NCBI) | 132720 |
| RRID | AB_3742977 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8N8J7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The FAM241A protein, also known as C4orf32, is a key regulatory factor that is localized to the Golgi apparatus. It plays an important role in maintaining the structural integrity of the Golgi apparatus and is involved in processes such as protein glycosylation and vesicle transport. More importantly, FAM241A has been identified as a novel negative regulator of the Hippo signaling pathway. It directly binds to and inhibits the activity of the core kinases MST1/2, thereby relieving the suppression of the downstream effectors YAP/TAZ, promoting cell proliferation, and inhibiting apoptosis. Aberrant function of FAM241A is closely related to the occurrence and development of various human diseases, especially tumors, making it a highly promising target for basic research and clinical translation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C4orf32 antibody 33453-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
