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验证数据展示

  • IHC staining of human liver using 25036-1-AP

    IHC staining of human liver using 25036-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25036-1-AP (C4orf29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of PC-3 using 25036-1-AP

    WB analysis of PC-3 using 25036-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25036-1-AP (C4orf29 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 25036-1-AP

    IF Staining of A549 using 25036-1-AP

    Immunofluorescent analysis of A549 cells using 25036-1-AP (C4orf29 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

C4orf29 Polyclonal antibody

C4orf29 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25036-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

C4orf29

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, A549 cells, mouse heart tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25036-1-AP targets C4orf29 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 C4orf29 fusion protein Ag19120 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 4 open reading frame 29
别名 C4orf29
计算分子量 414 aa, 47 kDa
观测分子量 60 kDa
GenBank蛋白编号BC128143
基因名称 C4orf29
Gene ID (NCBI) 80167
RRIDAB_2879862
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ0P651
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C4orf29 (Chromosome 4 open reading frame 29) is a secreted protein in humans that is encoded by the C4orf29 gene which maps to human chromosome 4. The function of C4orf29 is uncharacterised.

实验方案

Product Specific Protocols
WB protocol for C4orf29 antibody 25036-1-APDownload protocol
IHC protocol for C4orf29 antibody 25036-1-APDownload protocol
IF protocol for C4orf29 antibody 25036-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols