验证数据展示
经过测试的应用
Positive WB detected in | human blood |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
55228-1-AP targets C4-B Alpha Chain/C4b-B/C4d-B in WB, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | complement component 4B (Chido blood group) |
别名 | C4, C4B, C4B1, C4B12, C4B2, C4B3, C4B5, C4F, CH, CO4, Complement C4-B, CPAMD3 |
计算分子量 | 193 kDa |
观测分子量 | 95 kDa, 300 kDa |
GenBank蛋白编号 | NM_000592 |
基因名称 | C4B |
Gene ID (NCBI) | 721 |
RRID | AB_2881291 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P0C0L5 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular wights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1116-1128aa of human C4-B protein can recognize C4-B precursor, C4-B alpha chain, C4b-B, and C4d-B.
实验方案
Product Specific Protocols | |
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WB protocol for C4-B Alpha Chain/C4b-B/C4d-B antibody 55228-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |