验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, NIH/3T3 cells, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27595-1-AP targets EOGT in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag25932 Product name: Recombinant human C3orf64 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 18-274 aa of BC060887 Sequence: GQNEAPPNTHSIPGEPLYNYASIRLPEEHIPFFLHNNRHIATVCRKDSLCPYKKHLEKLKYCWGYEKSCKPEFRFGYPVCSYVDMGWTDTLESAEDIFWKQADFGYARERLEEMHVLCQPKETSDSSLVCSRYLQYCRATNLYLDLRNIKRNHDRFKEDFFQSGEIGGHCKLDIRTLTSEGQRKSPLQSWFAELQSYTQLNFRPIEDAKCDIVIEKPTYFMKLDAGVNMYHHFCDFINLYITQHVNNSFSTDVYIVM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 3 open reading frame 64 |
| 别名 | C3orf64, AER61, EC:2.4.1.255, EGF domain-specific O-linked N-acetylglucosamine transferase, EOGT1 |
| 观测分子量 | 60-62 kDa |
| GenBank蛋白编号 | BC060887 |
| 基因名称 | C3orf64 |
| Gene ID (NCBI) | 285203 |
| RRID | AB_2880919 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5NDL2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EOGT (EGF Domain-Specific O-Linked N-Acetylglucosamine Transferase) is an endoplasmic reticulum-resident glycosyltransferase that catalyzes O-GlcNAc modification of serine/threonine residues in proteins containing EGF-like repeats, regulating protein folding, maturation, and secretion. EOGT-mediated O-GlcNAc modification is crucial for the functions of ligands and receptors in key developmental signaling pathways such as the Notch and Wnt signaling pathways, influencing cell fate determination, tissue development, and stem cell maintenance. Mutations in the EOGT gene cause congenital disorders of glycosylation, manifesting as neurodevelopmental abnormalities and skeletal malformations. Furthermore, in cell biology and developmental biology research, anti-EOGT antibodies are widely used for studying glycosylation mechanisms, Notch signaling pathway analysis, and exploring developmental disease models.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for EOGT antibody 27595-1-AP | Download protocol |
| IP protocol for EOGT antibody 27595-1-AP | Download protocol |
| WB protocol for EOGT antibody 27595-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
J Extracell Vesicles Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease | ||
Adv Sci (Weinh) Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia | ||
Genomics SHCBP1 interacting with EOGT enhances O-GlcNAcylation of NOTCH1 and promotes the development of pancreatic cancer.
| ||
Proteomics Solvent-induced proteome profiling for proteomic quantitation and target discovery of small molecular drugs | ||
Cartilage HSF1 Increases EOGT-Mediated Glycosylation of Notch1 to Promote IL-1β-Induced Inflammatory Injury of Chondrocytes
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