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验证数据展示

  • WB analysis of mouse heart using 25362-1-AP

    WB analysis of mouse heart using 25362-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25362-1-AP (C3orf43 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 25362-1-AP

    IF Staining of A431 using 25362-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C3orf43 antibody (25362-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of A431 using 25362-1-AP

    IF Staining of A431 using 25362-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using C3orf43 antibody (25362-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

C3orf43 Polyclonal antibody

C3orf43 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25362-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Single-pass membrane and coiled-coil domain-containing protein 1, Single-pass membrane protein with coiled-coil domains 1, SMCO1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue, human plasma tissue
Positive IF/ICC detected inA431 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25362-1-AP targets C3orf43 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag21670

Product name: Recombinant human C3orf43 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-214 aa of BC118636

Sequence: MNNETTTLISLKEAMKRVDHKLQALETQFKELDFTKDNLMQKFEHHSKALASQAAQDEMWTAVRALQLTSMELNILYSYVIEVLICLHTRVLEKLPDLVRGLPTLASVLRRKVKNKRVRVVWESILEECGLQEGDITALCTFFIARGNKAEHYTAKVRQMYIRDVTFLITNMVKNQALQDSLLRAVQVIEKGKAVRTPEKQKSSLEELIPSVKN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 3 open reading frame 43
别名 Single-pass membrane and coiled-coil domain-containing protein 1, Single-pass membrane protein with coiled-coil domains 1, SMCO1
计算分子量 214 aa, 25 kDa
观测分子量 25-30 kDa
GenBank蛋白编号BC118636
基因名称 C3orf43
Gene ID (NCBI) 255798
RRIDAB_2880044
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ147U7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for C3orf43 antibody 25362-1-APDownload protocol
WB protocol for C3orf43 antibody 25362-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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