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验证数据展示

  • WB analysis of mouse skeletal muscle using 25575-1-AP

    WB analysis of mouse skeletal muscle using 25575-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25575-1-AP (C3orf39 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • IP experiment of mouse skeletal muscle using 25575-1-AP

    IP experiment of mouse skeletal muscle using 25575-1-AP

    IP result of anti-C3orf39 (IP:25575-1-AP, 3ug; Detection:25575-1-AP 1:400) with mouse skeletal muscle tissue lysate 2500ug.

C3orf39 Polyclonal antibody

C3orf39 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25575-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

AGO61, C3orf39

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25575-1-AP targets C3orf39 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag22424

Product name: Recombinant human C3orf39 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 235-580 aa of BC060861

Sequence: GLSKITTWYQYGFVQPQGPKANILVSGNEIRQFARFMTEKLNVSHTGVPLGEEYILVFSRTQNRLILNEAELLLALAQEFQMKTVTVSLEDHTFADVVRLVSNASMLVSMHGAQLVTTLFLPRGATVVELFPYAVNPDHYTPYKTLAMLPGMDLQYVAWRNMMPENTVTHPERPWDQGGITHLDRAEQARILQSREVPRHLCCRNPEWLFRIYQDTKVDIPSLIQTIRRVVKGRPGPRKQKWTVGLYPGKVREARCQASVHGASEARLTVSWQIPWNLKYLKVREVKYEVWLQEQGENTYVPYILALQNHTFTENIKPFTTYLVWVRCIFNKILLGPFADVLVCNT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 3 open reading frame 39
别名 AGO61, C3orf39
计算分子量 580 aa, 67 kDa
观测分子量 67-70 kDa
GenBank蛋白编号BC060861
基因名称 C3orf39
Gene ID (NCBI) 84892
RRIDAB_2880135
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NAT1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C3orf39 antibody 25575-1-APDownload protocol
IP protocol for C3orf39 antibody 25575-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols