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C3orf31 Polyclonal antibody

C3orf31 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 28137-1-AP

Print datasheet

Synonyms

C3orf31, DKFZp434E0519

验证数据展示

  • WB analysis of LNCaP using 28137-1-AP

    WB analysis of LNCaP using 28137-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 28137-1-AP (C3orf31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 28137-1-AP

    IHC staining of human kidney using 28137-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28137-1-AP (C3orf31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inLNCaP cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28137-1-AP targets C3orf31 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB,ELISA
Tested Reactivity Human
Immunogen C3orf31 fusion protein Ag26826 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 3 open reading frame 31
Synonyms C3orf31, DKFZp434E0519
Calculated Molecular Weight 316 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC015088
Gene Symbol C3orf31
Gene ID (NCBI) 132001
RRIDAB_2881071
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96BW9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C3orf31 antibody 28137-1-APDownload protocol
IHC protocol for C3orf31 antibody 28137-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols