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验证数据展示

  • WB analysis of human plasma using 87386-1-RR (same clone as 87386-1-PBS)

    WB analysis of human plasma using 87386-1-RR (same clone as 87386-1-PBS)

    Human plasma was subjected to SDS PAGE followed by western blot with 87386-1-RR (Complement component C3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87386-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 87386-1-RR (same clone as 87386-1-PBS)

    IHC staining of human liver cancer using 87386-1-RR (same clone as 87386-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 87386-1-RR (C3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87386-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with C3 antibody included
  • Affinity of 87386-1-RR

    Affinity of 87386-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87386-1-RR against Human C3 were performed. The affinity constant is below 1 pM.

C3 Recombinant monoclonal antibody, PBS Only

C3 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 87386-1-PBS

产品说明书

CloneNo. 252159E1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

C3/C3a, C3/C3b/C3c, Acylation stimulating protein, ARMD9, ASP

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy C3 Ready-To-Use IHC Kit. C3 primary antibody included.

产品信息

87386-1-PBS targets C3 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg4880

Product name: Recombinant human Complement component C3 protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 672-1663 aa of NM_000064.4

Sequence: SVQLTEKRMDKVGKYPKELRKCCEDGMRENPMRFSCQRRTRFISLGEACKKVFLDCCNYITELRRQHARASHLGLARSNLDEDIIAEENIVSRSEFPESWLWNVEDLKEPPKNGISTKLMNIFLKDSITTWEILAVSMSDKKGICVADPFEVTVMQDFFIDLRLPYSVVRNEQVEIRAVLYNYRQNQELKVRVELLHNPAFCSLATTKRRHQQTVTIPPKSSLSVPYVIVPLKTGLQEVEVKAAVYHHFISDGVRKSLKVVPEGIRMNKTVAVRTLDPERLGREGVQKEDIPPADLSDQVPDTESETRILLQGTPVAQMTEDAVDAERLKHLIVTPSGCGEQNMIGMTPTVIAVHYLDETEQWEKFGLEKRQGALELIKKGYTQQLAFRQPSSAFAAFVKRAPSTWLTAYVVKVFSLAVNLIAIDSQVLCGAVKWLILEKQKPDGVFQEDAPVIHQEMIGGLRNNNEKDMALTAFVLISLQEAKDICEEQVNSLPGSITKAGDFLEANYMNLQRSYTVAIAGYALAQMGRLKGPLLNKFLTTAKDKNRWEDPGKQLYNVEATSYALLALLQLKDFDFVPPVVRWLNEQRYYGGGYGSTQATFMVFQALAQYQKDAPDHQELNLDVSLQLPSRSSKITHRIHWESASLLRSEETKENEGFTVTAEGKGQGTLSVVTMYHAKAKDQLTCNKFDLKVTIKPAPETEKRPQDAKNTMILEICTRYRGDQDATMSILDISMMTGFAPDTDDLKQLANGVDRYISKYELDKAFSDRNTLIIYLDKVSHSEDDCLAFKVHQYFNVELIQPGAVKVYAYYNLEESCTRFYHPEKEDGKLNKLCRDELCRCAEENCFIQKSDDKVTLEERLDKACEPGVDYVYKTRLVKVQLSNDFDEYIMAIEQTIKSGSDEVQVGQQRTFISPIKCREALKLEEKKHYLMWGLSSDFWGEKPNLSYIIGKDTWVEHWPEEDECQDEENQKQCQDLGAFTESMVVFGCPN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 complement component 3
别名 C3/C3a, C3/C3b/C3c, Acylation stimulating protein, ARMD9, ASP
计算分子量187 kDa
观测分子量115-120 kDa
GenBank蛋白编号NM_000064.4
基因名称 C3
Gene ID (NCBI) 718
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP01024
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The complement system is an important effector that bridges the innate and adaptive immune systems (PMID: 20010915). The third component of complement, C3, plays a central role in activating the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways (PMID: 11414361). Human C3 (190-195 kDa), composed of α and β chains (115-120 and 75 kDa, respectively), is cleaved into C3a and C3b by C3 convertase. C3b is composed of the α' chain and β chain (PMID: 27210597). Factor I cleaves the α′ chain of C3b to 68 kDa and 43 kDa degradation products (iC3b) (PMID: 25395424; 14527961). This antibody was raised against 672-1663 aa of the human C3 protein.

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