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验证数据展示

  • IHC staining of human kidney using 24930-1-AP

    IHC staining of human kidney using 24930-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24930-1-AP (C2orf47 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse kidney using 24930-1-AP

    WB analysis of mouse kidney using 24930-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24930-1-AP (C2orf47 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

C2orf47 Polyclonal antibody

C2orf47 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24930-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

m-AAA protease-interacting protein 1, mitochondrial, MAIP1, Matrix AAA peptidase-interacting protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse kidney tissue, HeLa cells, PC-3 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

24930-1-AP targets C2orf47 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原

CatNo: Ag21518

Product name: Recombinant human C2orf47 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-291 aa of BC017959

Sequence: MALAARLLPQFLHSRSLPCGAVRLRTPAVAEVRLPSATLCYFCRCRLGLGAALFPRSARALAASALPAQGSRWPVLSSPGLPAAFASFPACPQRSYSTEEKPQQHQKTKMIVLGFSNPINWVRTRIKAFLIWAYFDKEFSITEFSEGAKQAFAHVSKLLSQCKFDLLEELVAKEVLHALKEKVTSLPDNHKNALAANIDEIVFTSTGDISIYYDEKGRKFVNILMCFWYLTSANIPSETLRGASVFQVKLGNQNVETKQLLSASYEFQREFTQGVKPDWTIARIEHSKLLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 2 open reading frame 47
别名 m-AAA protease-interacting protein 1, mitochondrial, MAIP1, Matrix AAA peptidase-interacting protein 1
计算分子量 291 aa, 33 kDa
观测分子量 38 kDa
GenBank蛋白编号BC017959
基因名称 C2orf47
Gene ID (NCBI) 79568
RRIDAB_2879804
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WWC4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C2orf47, also named matrix AAA peptidase interacting protein 1 (MAIP1), is a 291 amino-acid protein that localizes in the Mitochondrion. C2orf47 is involved in protecting EMRE, a component of the single calcium complex (MCU), from proteolysis by assisting membrane insertion.

实验方案

Product Specific Protocols
WB protocol for C2orf47 antibody 24930-1-APDownload protocol
IHC protocol for C2orf47 antibody 24930-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB

Nat Cell Biol

Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space

Authors - Elias Adriaenssens
View Article