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C2orf37 Polyclonal antibody

C2orf37 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 26033-1-AP

Print datasheet

Synonyms

C2orf37, DCAF17

验证数据展示

  • WB analysis of HEK-293 using 26033-1-AP

    WB analysis of HEK-293 using 26033-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 26033-1-AP (C2orf37 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 26033-1-AP

    IHC staining of mouse liver using 26033-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26033-1-AP (C2orf37 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 26033-1-AP

    IHC staining of mouse liver using 26033-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26033-1-AP (C2orf37 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HL-60 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26033-1-AP is C2orf37 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse
Immunogen C2orf37 fusion protein Ag21995 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 2 open reading frame 37
Synonyms C2orf37, DCAF17
Calculated Molecular Weight 520 aa, 59 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC140843
Gene Symbol C2orf37
Gene ID (NCBI) 80067
RRIDAB_2880344
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5H9S7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C2orf37 antibody 26033-1-APDownload protocol
IHC protocol for C2orf37 antibody 26033-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols