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验证数据展示

  • IF Staining of U2OS using 26577-1-AP

    IF Staining of U2OS using 26577-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 26577-1-AP (C22orf33 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of human testis using 26577-1-AP

    WB analysis of human testis using 26577-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 26577-1-AP (C22orf33 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 26577-1-AP

    IHC staining of mouse testis using 26577-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26577-1-AP (C22orf33 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 26577-1-AP

    IF Staining of mouse testis using 26577-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using C22orf33 antibody (26577-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

C22orf33 Polyclonal antibody

C22orf33 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 26577-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

cE81G9.2, Ciliary microtubule inner protein 4, CIMIP4, EAN57, Protein EAN57

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26577-1-AP targets C22orf33 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag23900

Product name: Recombinant human C22orf33 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-195 aa of BC042635

Sequence: MDPQSRSLKNAGSRSSSRENRATSGEGAQPCQGTDDGPSLGAQDQRSTPTNQKGSIIPNNIRHKFGSNVVDQLVSEEQAQKAIDEVFEGQKRASSWPSRTQNPVEISSVFSDYYDLGYNMRSNLFRGAAEETKSLMKASYTPEVIEKSVRDLEHWHGRKTDDLGRWHQKNAMNLNLQKALEEKYGENSKSKSSKY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 22 open reading frame 33
别名 cE81G9.2, Ciliary microtubule inner protein 4, CIMIP4, EAN57, Protein EAN57
观测分子量 31 kDa
GenBank蛋白编号BC042635
基因名称 C22orf33
Gene ID (NCBI) 339669
RRIDAB_2880558
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43247
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for C22orf33 antibody 26577-1-APDownload protocol
IHC protocol for C22orf33 antibody 26577-1-APDownload protocol
WB protocol for C22orf33 antibody 26577-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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