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验证数据展示

  • WB analysis using 86258-4-RR (same clone as 86258-4-PBS)

    WB analysis using 86258-4-RR (same clone as 86258-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86258-4-RR (C22orf28 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86258-4-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 86258-4-RR (same clone as 86258-4-PBS)

    IF Staining of Jurkat using 86258-4-RR (same clone as 86258-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using RTCB antibody (86258-4-RR, Clone: 250866B2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86258-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86258-4-PBS

    Affinity and Kinetic Characterization of 86258-4-PBS

    Biolayer interferometry (BLl) kinetic assays of 86258-4-PBS against Human RTCB were performed. The affinity constant is 0.303 nM.

RTCB Recombinant monoclonal antibody, PBS Only

RTCB Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86258-4-PBS

产品说明书

CloneNo. 250866B2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

HSPC117, 3'-phosphate/5'-hydroxy nucleic acid ligase, C22orf28, EC:6.5.1.8, FAAP

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RTCB Ready-To-Use IHC Kit. RTCB primary antibody included.

产品信息

86258-4-PBS targets RTCB in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13836

Product name: Recombinant human C22orf28 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 159-505 aa of BC000151

Sequence: GVIPMNAKDLEEALEMGVDWSLREGYAWAEDKEHCEEYGRMLQADPNKVSARAKKRGLPQLGTLGAGNHYAEIQVVDEIFNEYAAKKMGIDHKGQVCVMIHSGSRGLGHQVATDALVAMEKAMKRDKIIVNDRQLACARIASPEGQDYLKGMAAAGNYAWVNRSSMTFLTRQAFAKVFNTTPDDFDLHVIYDVSHNIAKVEQHVVDGKERTLLVHRKGSTRAFPPHHPLIAVDYQLTGQPVLIGGTMGTCSYVLTGTEQGMTETFGTTCHGAGRALSRAKSRRNLDFQDVLDKLADMGIAIRVASPKLVMEEAPESYKNVTDVVNTCHDAGISKKAIKLRPIAVIKG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 22 open reading frame 28
别名 HSPC117, 3'-phosphate/5'-hydroxy nucleic acid ligase, C22orf28, EC:6.5.1.8, FAAP
计算分子量 55 kDa
观测分子量55 kDa
GenBank蛋白编号BC000151
基因名称 RTCB
Gene ID (NCBI) 51493
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y3I0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RTCB (also known as HSPC117, C22orf28, FAAP and D10Wsu52e) is an essential subunit of a tRNA ligase complex that is involved in RNA repair and stress-induced splicing. As a multifunctional protein, RTCB is also a cell adhesion protein and is important in embryo and placenta development. Recently RTCB has been identified as UPR RNA ligase catalyzing unconventional XBP1 mRNA splicing. This antibody specifically recognizes endogenous RTCB protein. (25087875)

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