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RtcB-Specific Polyclonal antibody
RtcB-Specific Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, mouse brain tissue, Jurkat cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human lung cancer tissue, human liver cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
19809-1-AP targets RtcB-Specific in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, pig |
Immunogen | RtcB-Specific fusion protein Ag13836 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | chromosome 22 open reading frame 28 |
Synonyms | C22orf28, FAAP, focal adhesion associated protein, hematopoietic stem/progenitor cell 117, HSPC117, RtcB, RtcB-Specific |
Calculated Molecular Weight | 55 kDa |
Observed Molecular Weight | 55 kDa |
GenBank Accession Number | BC000151 |
Gene Symbol | C22orf28 |
Gene ID (NCBI) | 51493 |
RRID | AB_10695047 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y3I0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for RtcB-Specific antibody 19809-1-AP | Download protocol |
IHC protocol for RtcB-Specific antibody 19809-1-AP | Download protocol |
IF protocol for RtcB-Specific antibody 19809-1-AP | Download protocol |
IP protocol for RtcB-Specific antibody 19809-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Reprogramming of microRNA expression via E2F1 downregulation promotes Salmonella infection both in infected and bystander cells. | ||
J Biol Chem The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonical XBP1 splicing during ER stress.
| ||
Life Sci Alliance Stress-induced tyrosine phosphorylation of RtcB modulates IRE1 activity and signaling outputs. | ||
J Immunol The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1 |