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验证数据展示

  • WB analysis of human brain using 21357-1-AP

    WB analysis of human brain using 21357-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21357-1-AP (C21orf66 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of Raji using 21357-1-AP

    IP experiment of Raji using 21357-1-AP

    IP result of anti-C21orf66 (IP:21357-1-AP, 4ug; Detection:21357-1-AP 1:500) with Raji cells lysate 1440 ug.

  • IHC staining of mouse liver using 21357-1-AP

    IHC staining of mouse liver using 21357-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 21357-1-AP

    IHC staining of human pancreas cancer using 21357-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human renal cell carcinoma using 21357-1-AP

    IHC staining of human renal cell carcinoma using 21357-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human renal cell carcinoma using 21357-1-AP

    IHC staining of human renal cell carcinoma using 21357-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 21357-1-AP

    IF Staining of HeLa using 21357-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using C21orf66 antibody (21357-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

C21orf66 Polyclonal antibody

C21orf66 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21357-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

BM 020, fSAP105, GCFC, GCFC1, GC-rich sequence DNA-binding factor 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman brain tissue
Positive IP detected inRaji cells
Positive IHC detected inhuman renal cell carcinoma tissue, human pancreas cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21357-1-AP targets C21orf66 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

CatNo: Ag15789

Product name: Recombinant human C21orf66 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC030539

Sequence: MFRKARRVNVRKRNDSEEEERERDEEQEPPPLLPPPGTGEEAGPGGGDRAPGGESLLGPGPSPPSALTPGLGAEAGGGFPGGAEPGNGLKPRKRPRENKEVPRASLLSFQDEEEENEEVFKVKKSSYSKKIVKLLKKEYKEDLEKSKIKTELNSSAESEQPLDKTGHVKDTNQEDGVIISEHGEDEMDMESEKEEEKPKTGGAFSNALSSLNVLRPGEIPDAAFIHAARKKRQMARELGDFTPHDNEPGKGRLVREDENDASDDEDDDEKRRIVFSVKEKSQRQKIAEEIGIEGSDDDALVTGEQDEELSRWEQEQIRKGINIPQVQASQPAEVNMYYQNTYQTMPYGSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 21 open reading frame 66
别名 BM 020, fSAP105, GCFC, GCFC1, GC-rich sequence DNA-binding factor 1
计算分子量 917 aa, 105 kDa
观测分子量 93 kDa
GenBank蛋白编号BC030539
基因名称 C21orf66
Gene ID (NCBI) 94104
RRIDAB_10733239
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5B6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for C21orf66 antibody 21357-1-APDownload protocol
IHC protocol for C21orf66 antibody 21357-1-APDownload protocol
IP protocol for C21orf66 antibody 21357-1-APDownload protocol
WB protocol for C21orf66 antibody 21357-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Immunol

Paxbp1 is indispensable for the survival of CD4 and CD8 double-positive thymocytes

Authors - Wenting Li
View Article
mouseIHC

J Invest Dermatol

Paxbp1 is indispensable for the maintenance of epidermal homeostasis

Authors - Cong Huang
View Article
mouseWB

Immunology

Paxbp1 is indispensable for the maintenance of peripheral CD4 T cell homeostasis

Authors - Wenting Li
View Article
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