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验证数据展示

  • WB analysis of K-562 using 21461-1-AP

    WB analysis of K-562 using 21461-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 21461-1-AP (C21orf59 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 21461-1-AP

    IF Staining of HEK-293 using 21461-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using C21orf59 antibody (21461-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

C21orf59 Polyclonal antibody

C21orf59 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21461-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Protein kurly homolog, C21orf48

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, MCF-7 cells, PC-3 cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

21461-1-AP targets C21orf59 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 C21orf59 fusion protein Ag14994 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 21 open reading frame 59
别名 Protein kurly homolog, C21orf48
计算分子量 290 aa, 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC000709
基因名称 C21orf59
Gene ID (NCBI) 56683
RRIDAB_2878863
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP57076
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C21orf59 antibody 21461-1-APDownload protocol
IF protocol for C21orf59 antibody 21461-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

FACS-based genome-wide CRISPR screens define key regulators of DNA damage signaling pathways

Authors - Min Huang
View Article