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验证数据展示

  • WB analysis using 27609-1-AP

    WB analysis using 27609-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 27609-1-AP (C21orf2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 27609-1-AP

    IHC staining of human lung cancer using 27609-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27609-1-AP (C21orf2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HEK-293T using 27609-1-AP

    IP experiment of HEK-293T using 27609-1-AP

    IP result of anti-C21orf2 (IP:27609-1-AP, 4ug; Detection:27609-1-AP 1:3000) with HEK-293T cells lysate 1320 ug.

C21orf2 Polyclonal antibody

C21orf2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 27609-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

Human and More (1)

应用

WB, IP, IHC, ELISA

别名

A2, C21orf HUMF09G8.5, C21orf2, YF5, YF5/A2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells
Positive IP detected inHEK-293T cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27609-1-AP targets C21orf2 in WB, IHC, IP, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IP
经测试反应性 Human
文献引用反应性human
免疫原 C21orf2 fusion protein Ag26470 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 21 open reading frame 2
别名 A2, C21orf HUMF09G8.5, C21orf2, YF5, YF5/A2
观测分子量 28-32 kDa
GenBank蛋白编号BC072012
基因名称 C21orf2
Gene ID (NCBI) 755
RRIDAB_2880924
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43822
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C21orf2 antibody 27609-1-APDownload protocol
IHC protocol for C21orf2 antibody 27609-1-APDownload protocol
IP protocol for C21orf2 antibody 27609-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Life Sci Alliance

Functional characterization of C21ORF2 association with the NEK1 kinase mutated in human in diseases

Authors - Mateusz Gregorczyk
View Article
humanWB

Acta Neuropathol Commun

ALS-associated C21ORF2 variant disrupts DNA damage repair, mitochondrial metabolism, neuronal excitability and NEK1 levels in human motor neurons

Authors - Pavol Zelina
View Article