验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells |
| Positive IP detected in | HEK-293T cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IP | See 1 publications below |
产品信息
27609-1-AP targets C21orf2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag26470 Product name: Recombinant human C21orf2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-256 aa of BC072012 Sequence: MKLTRKMVLTRAKASELHSVRKLNCWGSRLTDISICQEMPSLEVITLSVNSISTLEPVSRCQRLSELYLRRNRIPSLAELFYLKGLPRLRVLWLAENPCCGTSPHRYRMTVLRTLPRLQKLDNQAVTEEELSRALSEGEEITAAPEREGTGHGGPKLCCTLSSLSSAAETGRDPLDSEEEATSGAQDERGLKPPSRGQFPSLSARDASSSHRGRNVLTAILLLLRELDAEGLEAVQQTVGSRLQALRGEEVQEHAE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 21 open reading frame 2 |
| 别名 | A2, C21orf-HUMF09G8.5, CFAP410, Cilia- and flagella-associated protein 410, Leucine-rich repeat-containing protein 76 |
| 观测分子量 | 28-32 kDa |
| GenBank蛋白编号 | BC072012 |
| 基因名称 | C21orf2 |
| Gene ID (NCBI) | 755 |
| RRID | AB_2880924 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43822 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for C21orf2 antibody 27609-1-AP | Download protocol |
| IP protocol for C21orf2 antibody 27609-1-AP | Download protocol |
| WB protocol for C21orf2 antibody 27609-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Life Sci Alliance Functional characterization of C21ORF2 association with the NEK1 kinase mutated in human in diseases | ||
Acta Neuropathol Commun ALS-associated C21ORF2 variant disrupts DNA damage repair, mitochondrial metabolism, neuronal excitability and NEK1 levels in human motor neurons |
