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验证数据展示

  • WB analysis of mouse brain using 25626-1-AP

    WB analysis of mouse brain using 25626-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25626-1-AP (SLC52A3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 25626-1-AP

    IHC staining of human small intestine using 25626-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25626-1-AP (SLC52A3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C20orf54/SLC52A3 antibody included
  • IHC staining of human oesophagus cancer using 25626-1-AP

    IHC staining of human oesophagus cancer using 25626-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 25626-1-AP (SLC52A3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C20orf54/SLC52A3 antibody included
  • IHC staining of human stomach cancer using 25626-1-AP

    IHC staining of human stomach cancer using 25626-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25626-1-AP (SLC52A3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C20orf54/SLC52A3 antibody included
  • IHC staining of human stomach cancer using 25626-1-AP

    IHC staining of human stomach cancer using 25626-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25626-1-AP (SLC52A3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C20orf54/SLC52A3 antibody included

SLC52A3 Polyclonal antibody

SLC52A3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25626-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

C20orf54, hRFT2, RFT2, RFVT3, Riboflavin transporter 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman stomach cancer tissue, human oesophagus cancer tissue, human small intestine tissue, human testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy C20orf54/SLC52A3 Ready-To-Use IHC Kit. C20orf54/SLC52A3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25626-1-AP targets SLC52A3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SLC52A3 fusion protein Ag22315 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Solute carrier family 52, riboflavin transporter, member 3
别名 C20orf54, hRFT2, RFT2, RFVT3, Riboflavin transporter 2
计算分子量 469 aa, 51 kDa
观测分子量52 kDa
GenBank蛋白编号BC009750
基因名称 SLC52A3
Gene ID (NCBI) 113278
RRIDAB_2880167
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQ40
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLC52A3 antibody 25626-1-APDownload protocol
IHC protocol for SLC52A3 antibody 25626-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols