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验证数据展示

  • WB analysis of HepG2 using 25953-1-AP

    WB analysis of HepG2 using 25953-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25953-1-AP (C20orf3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 25953-1-AP

    IP experiment of mouse liver using 25953-1-AP

    IP result of anti-C20orf3/APMAP (IP:25953-1-AP, 4ug; Detection:25953-1-AP 1:300) with mouse liver tissue lysate 4800ug.

  • IHC staining of human kidney using 25953-1-AP

    IHC staining of human kidney using 25953-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25953-1-AP (C20orf3 Antibody) at dilution of 1:200 (under 40x lens).

  • Featured Product
  • KD/KO验证

C20orf3/APMAP Polyclonal antibody

C20orf3/APMAP Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 25953-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, ELISA and More (1)

别名

C20orf3, UNQ1869/PRO4305, Protein BSCv, BSCv, APMAP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25953-1-AP targets C20orf3/APMAP in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 C20orf3/APMAP fusion protein Ag23029 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 20 open reading frame 3
别名 C20orf3, UNQ1869/PRO4305, Protein BSCv, BSCv, APMAP
计算分子量 416 aa, 46 kDa
观测分子量 42-46 kDa
GenBank蛋白编号BC003501
基因名称 APMAP
Gene ID (NCBI) 57136
RRIDAB_2880308
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HDC9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C20orf3/APMAP antibody 25953-1-APDownload protocol
IHC protocol for C20orf3/APMAP antibody 25953-1-APDownload protocol
IP protocol for C20orf3/APMAP antibody 25953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

Cancer Res

Cholesterol induces epithelial-to-mesenchymal transition of prostate cancer cells by suppressing degradation of EGFR through APMAP.

Authors - Siyuan Jiang
  • KD Validated
View Article
mouseWB

J Nutr Biochem

Thioredoxin domain containing 5 is involved in the hepatic storage of squalene into lipid droplets in a sex-specific way

Authors - Javier Sánchez-Marco
View Article