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验证数据展示

  • WB analysis of HepG2 using 26040-1-AP (same clone as 26040-1-PBS)

    WB analysis of HepG2 using 26040-1-AP (same clone as 26040-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26040-1-AP (C20orf20 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26040-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 26040-1-AP (same clone as 26040-1-PBS)

    IP experiment of HepG2 using 26040-1-AP (same clone as 26040-1-PBS)

    IP result of anti-C20orf20 (IP:26040-1-AP, 4ug; Detection:26040-1-AP 1:300) with HepG2 cells lysate 2640ug. This data was developed using the same antibody clone with 26040-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 26040-1-AP (same clone as 26040-1-PBS)

    IHC staining of human colon cancer using 26040-1-AP (same clone as 26040-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26040-1-AP (C20orf20 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26040-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 26040-1-AP (same clone as 26040-1-PBS)

    IHC staining of human colon cancer using 26040-1-AP (same clone as 26040-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26040-1-AP (C20orf20 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26040-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 26040-1-AP (same clone as 26040-1-PBS)

    IF Staining of HepG2 using 26040-1-AP (same clone as 26040-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C20orf20 antibody (26040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 26040-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

C20orf20 Polyclonal antibody, PBS Only

C20orf20 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 26040-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Eaf7, MRG/MORF4L-binding protein, MRG15BP, MRG-binding protein, MRGBP

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

26040-1-PBS targets C20orf20 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag22840

Product name: Recombinant human C20orf20 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-204 aa of BC014235

Sequence: MGEAEVGGGGAAGDKGPGEAATSPAEETVVWSPEVEVCLFHAMLGHKPVGVNRHFHMICIRDKFSQNIGRQVPSKVIWDHLSTMYDMQALHESEILPFPNPERNFVLPEEIIQEVREGKVMIEEEMKEEMKEDVDPHNGADDVFSSSGSLGKASEKSSKDKEKNSSDLGCKEGADKRKRSRVTDKVLTANSNPSSPSAAKRRRT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 20 open reading frame 20
别名 Eaf7, MRG/MORF4L-binding protein, MRG15BP, MRG-binding protein, MRGBP
计算分子量 204 aa, 22 kDa
观测分子量 30 kDa
GenBank蛋白编号BC014235
基因名称 C20orf20
Gene ID (NCBI) 55257
RRIDAB_2880347
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NV56
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

C20orf20, also named as MRG/MORF4L-binding protein or Up-regulated in colon cancer 4 (Urcc4), is a 204 amino acid protein, which belongs to the EAF7 family. C20orf20 localizes in the nucleus and is a Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. C20orf20 is modified through phosphorylation and acetylation, so the molecular weight of C20orf20 might be 25-30 kDa.

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