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C20orf195 Polyclonal antibody

C20orf195 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 16040-1-AP

Print datasheet

Synonyms

C20orf195

验证数据展示

  • WB analysis of HepG2 using 16040-1-AP

    WB analysis of HepG2 using 16040-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16040-1-AP (C20orf195 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • WB analysis of SMMC-7721 using 16040-1-AP

    WB analysis of SMMC-7721 using 16040-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16040-1-AP (C20orf195 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 16040-1-AP

    IHC staining of human prostate cancer using 16040-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16040-1-AP (C20orf195 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human prostate cancer using 16040-1-AP

    IHC staining of human prostate cancer using 16040-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16040-1-AP (C20orf195 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 16040-1-AP

    IF Staining of HepG2 using 16040-1-AP

    Immunofluorescent analysis of HepG2 cells using 16040-1-AP (C20orf195 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, SMMC-7721 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16040-1-AP targets C20orf195 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen C20orf195 fusion protein Ag8914 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 20 open reading frame 195
Synonyms C20orf195
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC000912
Gene Symbol C20orf195
Gene ID (NCBI) 79025
RRIDAB_2878209
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BVV2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C20orf195 antibody 16040-1-APDownload protocol
IHC protocol for C20orf195 antibody 16040-1-APDownload protocol
IF protocol for C20orf195 antibody 16040-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols