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验证数据展示

  • WB analysis of mouse brain using 23746-1-AP

    WB analysis of mouse brain using 23746-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 23746-1-AP (C20orf112 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 23746-1-AP

    IHC staining of human ovary cancer using 23746-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 23746-1-AP (C20orf112 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

C20orf112 Polyclonal antibody

C20orf112 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23746-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Nucleolar protein 4-like, NOL4L, C20orf113

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23746-1-AP targets C20orf112 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20690

Product name: Recombinant human C20orf112 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 40-166 aa of BC065370

Sequence: SSESGSGNGSSTLNPSTSSSTQGDPAFPEMNGNGAVAPMDFTTAAEDQPINLCDKLPPATALGTASYPSDGCGADGLRSRVKYGVKTTPESPPYSSGSYDSIKTEVSGCPEDLTVGRAPTADDDDDD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 20 open reading frame 112
别名 Nucleolar protein 4-like, NOL4L, C20orf113
计算分子量 436 aa, 47 kDa
观测分子量 43 kDa
GenBank蛋白编号BC065370
基因名称 C20orf112
Gene ID (NCBI) 140688
RRIDAB_2879315
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ96MY1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C20orf112 ,also termed as chromosome 20 open reading frame 112, is a 436 amino acid protein, which consists of a relatively hydrophobic N terminal region and two putative small coiled-coil domains. C20orf112 may likely involve in endocytosis pathway and normal brain development. It also is required to mediate the cell energy response. The function of C20orf112 is still non-known and mechanism is required to further study.

实验方案

Product Specific Protocols
IHC protocol for C20orf112 antibody 23746-1-APDownload protocol
WB protocol for C20orf112 antibody 23746-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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