验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, K-562 cells, HeLa cells, Jurkat cells, mouse brain tissue, rat brain tissue, SW480 cells, HepG2 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy GID8 Ready-To-Use IHC Kit. GID8 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24479-1-AP targets GID8 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | GID8 fusion protein Ag19751 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 20 open reading frame 11 |
| 别名 | C20orf11, GID8, Protein C20orf11, TWA1 |
| 计算分子量 | 228 aa, 27 kDa |
| 观测分子量 | 30 kDa |
| GenBank蛋白编号 | BC032120 |
| 基因名称 | GID8 |
| Gene ID (NCBI) | 54994 |
| RRID | AB_2879562 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NWU2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GID8 antibody 24479-1-AP | Download protocol |
| IHC protocol for GID8 antibody 24479-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS Genet Role of Bicaudal C1 in renal gluconeogenesis and its novel interaction with the CTLH complex. | ||
Sci Rep Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin. | ||
bioRxiv Genome-wide screen of Mycobacterium tuberculosis- infected macrophages identified the GID/CTLH complex as a determinant of intracellular bacterial growth | ||
Cancer Lett YTHDF1 regulates GID8-mediated glutamine metabolism to promote colorectal cancer progression in a m6A-dependent manner | ||
Mol Cell mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway | ||
Nat Commun Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth |
