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验证数据展示

  • WB analysis of human plasma using 85029-4-RR

    WB analysis of human plasma using 85029-4-RR

    human plasma was subjected to SDS PAGE followed by western blot with 85029-4-RR (C1R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 85029-4-RR

    IHC staining of mouse liver using 85029-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85029-4-RR (C1r antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C1R antibody included
  • IHC staining of mouse liver using 85029-4-RR

    IHC staining of mouse liver using 85029-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85029-4-RR (C1r antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C1R antibody included
  • Affinity and Kinetic Characterization of 85029-4-RR

    Affinity and Kinetic Characterization of 85029-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85029-4-RR against Human C1r were performed. The affinity constant is below 1 pM.

C1r Recombinant monoclonal antibody

C1r Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85029-4-RR

产品说明书

CloneNo. 242596F5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

242596F5, Complement C1r, Complement C1r subcomponent, Complement C1r subcomponent heavy chain, Complement C1r subcomponent light chain

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman plasma
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy C1R Ready-To-Use IHC Kit. C1R primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85029-4-RR targets C1r in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag11390

Product name: Recombinant human C1R protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 356-705 aa of BC035220

Sequence: AVCQDDGTWHRAMPRCKIKDCGQPRNLPNGDFRYTTTMGVNTYKARIQYYCHEPYYKMQTRAGSRESEQGVYTCTAQGIWKNEQKGEKIPRCLPVCGKPVNPVEQRQRIIGGQKAKMGNFPWQVFTNIHGRGGGALLGDRWILTAAHTLYPKEHEAQSNASLDVFLGHTNVEELMKLGNHPIRRVSVHPDYRQDESYNFEGDIALLELENSVTLGPNLLPICLPDNDTFYDLGLMGYVSGFGVMEEKIAHDLRFVRLPVANPQACENWLRGKNRMDVFSQNMFCAGHPSLKQDACQGDSGGVFAVRDPNTDRWVATGIVSWGIGCSRGYGFYTKVLNYVDWIKKEMEEED

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 complement component 1, r subcomponent
别名 242596F5, Complement C1r, Complement C1r subcomponent, Complement C1r subcomponent heavy chain, Complement C1r subcomponent light chain
计算分子量 705 aa, 80 kDa
观测分子量85 kDa
GenBank蛋白编号BC035220
基因名称 C1R
Gene ID (NCBI) 715
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP00736
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The first component of complement is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. The serine protease subunits C1r and C1s form a Ca(2+)-dependent C1s-C1r-C1r-C1s tetramer. C1r acts by transforming the activation signal into an enzymic activity. C1r is a single-chain glycoprotein that can be cleaved into an A chain and a B chain upon activation.

实验方案

Product Specific Protocols
IHC protocol for C1r antibody 85029-4-RRDownload protocol
WB protocol for C1r antibody 85029-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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