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验证数据展示

  • WB analysis using 20940-1-AP

    WB analysis using 20940-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20940-1-AP (C1orf83 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 20940-1-AP

    IF Staining of HeLa using 20940-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse brain using 20940-1-AP

    IHC staining of mouse brain using 20940-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 20940-1-AP

    IHC staining of mouse brain using 20940-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 20940-1-AP

    IHC staining of mouse cerebellum using 20940-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 20940-1-AP

    IHC staining of mouse cerebellum using 20940-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

C1orf83 Polyclonal antibody

C1orf83 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20940-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Transcription elongation factor A N-terminal and central domain-containing protein 2, TCEANC2, RP4 758J24.3, FLJ39169, FLJ32112

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IHC detected inmouse cerebellum tissue, human brain tissue, human breast cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20940-1-AP targets C1orf83 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 C1orf83 fusion protein Ag15040 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 1 open reading frame 83
别名 Transcription elongation factor A N-terminal and central domain-containing protein 2, TCEANC2, RP4 758J24.3, FLJ39169, FLJ32112
计算分子量 208 aa, 23 kDa
观测分子量 24 kDa
GenBank蛋白编号BC035374
基因名称 C1orf83
Gene ID (NCBI) 127428
RRIDAB_10860108
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96MN5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chromosome 1 open reading frame 83 (C1orf83) is also called TCEANC2, a little-studied transcription elongation factor. The rs10788972 variant of the TCEANC2 gene in the PARK10 region is closely associated with sporadic PD in the American population, while both rs10788972 and rs12046178 variants are not associated with sporadic PD in the Chinese Han population (PMID: 25663231; PMID: 26432391).

实验方案

Product Specific Protocols
WB protocol for C1orf83 antibody 20940-1-APDownload protocol
IHC protocol for C1orf83 antibody 20940-1-APDownload protocol
IF protocol for C1orf83 antibody 20940-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols