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验证数据展示

  • IHC staining of human breast cancer using 26109-1-AP

    IHC staining of human breast cancer using 26109-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26109-1-AP (C1orf58 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 26109-1-AP

    WB analysis using 26109-1-AP

    HEK293 cell lysates were subjected to SDS PAGE followed by western blot with 26109-1-AP (C1orf58 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 26109-1-AP

    IP experiment of HepG2 using 26109-1-AP

    IP result of anti-C1orf58 (IP:26109-1-AP, 4ug; Detection:26109-1-AP 1:300) with HepG2 cells lysate 1680 ug.

  • IHC staining of human breast cancer using 26109-1-AP

    IHC staining of human breast cancer using 26109-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26109-1-AP (C1orf58 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 26109-1-AP

    IF Staining of A431 using 26109-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C1orf58 antibody (26109-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • WB analysis of mouse brain using 26109-1-AP

    WB analysis of mouse brain using 26109-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26109-1-AP (C1orf58 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

C1orf58 Polyclonal antibody

C1orf58 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 26109-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

BROX, BROFTI, BRO1 domain-containing protein BROX, BRO1 domain containing protein BROX, BRO1 domain- and CAAX motif-containing protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26109-1-AP targets C1orf58 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag23443

Product name: Recombinant human C1orf58 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-355 aa of BC113635

Sequence: MTHWFHRNPLKATAPVSFNYYGVVTGPSASKICNDLRSSRARLLELFTDLSCNPEMMKNAADSYFSLLQGFINSLDESTQESKLRYIQNFKWTDTLQGQVPSAQQDAVFELISMGFNVALWYTKYASRLAGKENITEDEAKEVHRSLKIAAGIFKHLKESHLPKLITPAEKGRDLESRLIEAYVIQCQAEAQEVTIARAIELKHAPGLIAALAYETANFYQKADHTLSSLEPAYSAKWRKYLHLKMCFYTAYAYCYHGETLLASDKCGEAIRSLQEAEKLYAKAEALCKEYGETKGPGPTVKPSGHLFFRKLGNLVKNTLEKCQRENGFIYFQKIPTEAPQLELKANYGLVEPIP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 1 open reading frame 58
别名 BROX, BROFTI, BRO1 domain-containing protein BROX, BRO1 domain containing protein BROX, BRO1 domain- and CAAX motif-containing protein
观测分子量 46 kDa
GenBank蛋白编号BC113635
基因名称 C1orf58
Gene ID (NCBI) 148362
RRIDAB_2880382
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5VW32
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C1orf58 antibody 26109-1-APDownload protocol
IHC protocol for C1orf58 antibody 26109-1-APDownload protocol
IF protocol for C1orf58 antibody 26109-1-APDownload protocol
IP protocol for C1orf58 antibody 26109-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols