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C1orf50 Polyclonal antibody

C1orf50 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 20957-1-AP

Print datasheet

Synonyms

C1orf50

验证数据展示

  • WB analysis of HEK-293 using 20957-1-AP

    WB analysis of HEK-293 using 20957-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20957-1-AP (C1orf50 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 20957-1-AP

    IHC staining of human kidney using 20957-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20957-1-AP (C1orf50 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 20957-1-AP

    IHC staining of human kidney using 20957-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20957-1-AP (C1orf50 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293 cells, human testis tissue
Positive IHC detected inhuman kidney tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20957-1-AP is C1orf50 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse
Immunogen C1orf50 fusion protein Ag15124 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 1 open reading frame 50
Synonyms C1orf50
Calculated Molecular Weight 199 aa, 22 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC001711
Gene Symbol C1orf50
Gene ID (NCBI) 79078
RRIDAB_10733340
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BV19
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C1orf50 antibody 20957-1-APDownload protocol
IHC protocol for C1orf50 antibody 20957-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols