验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, COLO 320 cells, PC-3 cells |
| Positive IHC detected in | human placenta tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 1 publications below |
产品信息
25361-1-AP targets COA7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag19734 Product name: Recombinant human C1orf163 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-231 aa of BC015313 Sequence: MAGMVDFQDEEQVKSFLENMEVECNYHCYHEKDPDGCYRLVDYLEGIRKNFDEAAKVLKFNCEENQHSDSCYKLGAYYVTGKGGLTQDLKAAARCFLMACEKPGKKSIAACHNVGLLAHDGQVNEDGQPDLGKARDYYTRACDGGYTSSCFNLSAMFLQGAPGFPKDMDLACKYSMKACDLGHIWACANASRMYKLGDGVDKDEAKAEVLKNRAQQLHREQQKGVQPLTFG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 1 open reading frame 163 |
| 别名 | C1orf163, Beta-lactamase hcp-like protein, Cytochrome c oxidase assembly factor 7, RESA1, Respiratory chain assembly factor 1 |
| 计算分子量 | 231 aa, 26 kDa |
| 观测分子量 | 25-28 kDa |
| GenBank蛋白编号 | BC015313 |
| 基因名称 | COA7 |
| Gene ID (NCBI) | 65260 |
| RRID | AB_2880043 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96BR5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cytochrome c oxidase assembly factor 7 (COA7) is a 231 aa long mitochondrial protein present in animals, containing five Sel1-like tetratricopeptide repeat sequences, which are likely to interact with partner proteins (PMID: 30885959). COA7 is a metazoan-specific assembly factor, the absence or mutation of which in humans accompanies complex IV assembly defects and neurological conditions (PMID: 35210360). COA7 has a molecular weight of ~25 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for COA7 antibody 25361-1-AP | Download protocol |
| IHC protocol for COA7 antibody 25361-1-AP | Download protocol |
| WB protocol for COA7 antibody 25361-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Med Genet COA7 (C1orf163/RESA1) mutations associated with mitochondrial leukoencephalopathy and cytochrome c oxidase deficiency. | ||
Mol Biol Cell Discovery of bactericides as an acute mitochondrial membrane damage inducer. | ||
iScience Bisbiguanide analogs induce mitochondrial stress to inhibit lung cancer cell invasion |
