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验证数据展示

  • WB analysis of A431 using 25553-1-AP

    WB analysis of A431 using 25553-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 25553-1-AP (C1orf156 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 25553-1-AP

    IF Staining of A431 using 25553-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using METTL18 antibody (25553-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

METTL18 Polyclonal antibody

METTL18 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25553-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

C1orf156, Methyltransferase-like protein 18, Histidine protein methyltransferase 1 homolog, EC:2.1.1.85, AsTP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, PC-3 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

25553-1-AP targets METTL18 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 METTL18 fusion protein Ag22148 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 1 open reading frame 156
别名 C1orf156, Methyltransferase-like protein 18, Histidine protein methyltransferase 1 homolog, EC:2.1.1.85, AsTP2
计算分子量 372 aa, 42 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC008679
基因名称 METTL18
Gene ID (NCBI) 92342
RRIDAB_2880129
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95568
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL18 belongs to a family of protein lysine methyltransferases that methylate nonhistone proteins. Methyltransferases catalyze the transfer of a methyl group from a donor, generally S-adenosyl-L-methionine, to acceptor molecules, including proteins, DNA, RNA, lipids, and small metabolites. METTL18 is involved in peptidyl-lysine monomethylation.

实验方案

Product Specific Protocols
WB protocol for METTL18 antibody 25553-1-APDownload protocol
IF protocol for METTL18 antibody 25553-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Elife

METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance.

Authors - Eriko Matsuura-Suzuki
View Article
humanWB

Front Oncol

Identification METTL18 as a Potential Prognosis Biomarker and Associated With Immune Infiltrates in Hepatocellular Carcinoma.

Authors - Tian-Hao Li
View Article