验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, PC-3 cells |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
25553-1-AP targets METTL18 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag22148 Product name: Recombinant human C1orf156 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-372 aa of BC008679 Sequence: MTFQFNFTIEDHLENELTPIRDGALTLDSSKELSVSESQKGEERDRKCSAEQFDLPQDHLWEHKSMENAAPSQDTDSPLSAASSSRNLEPHGKQPSLRAAKEHAMPKDLKKMLENKVIETLPGFQHVKLSVVKTILLKENFPGENIVSKSFSSHSDLITGVYEGGLKIWECTFDLLAYFTKAKVKFAGKKVLDLGCGSGLLGITAFKGGSKEIHFQDYNSMVIDEVTLPNVVANSTLEDEENDVNEPDVKRCRKPKVTQLYKCRFFSGEWSEFCKLVLSSEKLFVKYDLILTSETIYNPDYYSNLHQTFLRLLSKNGRVLLASKAHYFGVGGGVHLFQKFVEERDVFKTRILKIIDEGLKRFIIEITFKFPG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 1 open reading frame 156 |
| 别名 | C1orf156, Arsenic-transactivated protein 2, AsTP2, EC:2.1.1.85, Histidine protein methyltransferase 1 homolog |
| 计算分子量 | 372 aa, 42 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC008679 |
| 基因名称 | METTL18 |
| Gene ID (NCBI) | 92342 |
| RRID | AB_2880129 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95568 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
METTL18 belongs to a family of protein lysine methyltransferases that methylate nonhistone proteins. Methyltransferases catalyze the transfer of a methyl group from a donor, generally S-adenosyl-L-methionine, to acceptor molecules, including proteins, DNA, RNA, lipids, and small metabolites. METTL18 is involved in peptidyl-lysine monomethylation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for METTL18 antibody 25553-1-AP | Download protocol |
| WB protocol for METTL18 antibody 25553-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance. | ||
Front Oncol Identification METTL18 as a Potential Prognosis Biomarker and Associated With Immune Infiltrates in Hepatocellular Carcinoma. |
