验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, mouse testis tissue |
| Positive IHC detected in | human stomach tissue, human brown disease Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
14888-1-AP targets C1orf116 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | C1orf116 fusion protein Ag6675 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 1 open reading frame 116 |
| 别名 | Specifically androgen-regulated gene protein, SARG |
| 计算分子量 | 64 kDa |
| 观测分子量 | 64 kDa, 38 kDa |
| GenBank蛋白编号 | BC000765 |
| 基因名称 | C1orf116 |
| Gene ID (NCBI) | 79098 |
| RRID | AB_2228225 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BW04 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Specifically androgen-regulated gene protein (SARG), encoded by C1orf116, is a 601-aa protein considered putative androgen-specific receptor. SARG mRNA is highly expressed in prostate and can be up-regulated by androgens, but not by glucocorticoids. Little is known about the function of SARG to date. Two isoforms of SARG are produced by alternative splicing encoding 64 kDa and 38 kDa proteins, and both isoforms can be recognized by this antibody 14888-1-AP.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C1orf116 antibody 14888-1-AP | Download protocol |
| IHC protocol for C1orf116 antibody 14888-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Signal Re-epithelialization of cancer cells increases autophagy and DNA damage: Implications for breast cancer dormancy and relapse | ||
Front Oncol The Specifically Androgen-Regulated Gene (SARG) Promotes Papillary Thyroid Carcinoma (PTC) Lymphatic Metastasis Through Vascular Endothelial Growth Factor C (VEGF-C) and VEGF Receptor 3 (VEGFR-3) Axis. |
