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验证数据展示

  • IHC staining of mouse testis using 25767-1-AP

    IHC staining of mouse testis using 25767-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25767-1-AP (C1orf113 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 25767-1-AP

    IHC staining of mouse testis using 25767-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25767-1-AP (C1orf113 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse testis using 25767-1-AP

    WB analysis of mouse testis using 25767-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25767-1-AP (C1orf113 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 25767-1-AP

    IP experiment of mouse testis using 25767-1-AP

    IP result of anti-C1orf113 (IP:25767-1-AP, 4ug; Detection:25767-1-AP 1:500) with mouse testis tissue lysate 4000ug.

C1orf113 Polyclonal antibody

C1orf113 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 25767-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

C1orf113, cDNA FLJ58582, Homo sapiens (Human), SH3D21

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25767-1-AP targets C1orf113 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag22643

Product name: Recombinant human C1orf113 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-342 aa of BC101676

Sequence: MPDKTATPERPPAPENAPSSKKIPAPDKVPSPEKTLTLGDKASIPGNSTSGKIPAPDKVPTPEKMVTPEDKASIPENSIIPEETLTVDKPSTPERVFSVEESPALEAPPMDKVPNPKMAPLGDEAPTLEKVLTPELSEEEVSTRDDIQFHHFSSEEALQKVKYFVAKEDPSSQEEAHTPEAPPPQPPSSERCLGEMKCTLVRGDSSPRQAELKSGPASRPALEKPHPHEEATTLPEEAPSNDERTPEEEAPPNEQRPLREEVLPKEGVASKEEVTLKEELPPKEEVAPKEEVPPIERAFAQKTRPIKPPPDSQETLALPSLVPQNYTENKNEGVDVTSLRGE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 1 open reading frame 113
别名 C1orf113, cDNA FLJ58582, Homo sapiens (Human), SH3D21
计算分子量 640 aa, 71 kDa
观测分子量 70-71 kDa
GenBank蛋白编号BC101676
基因名称 C1orf113
Gene ID (NCBI) 79729
RRIDAB_2880232
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDA4FU49
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C1orf113 antibody 25767-1-APDownload protocol
IHC protocol for C1orf113 antibody 25767-1-APDownload protocol
IP protocol for C1orf113 antibody 25767-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

PLoS One

Integrating bulk and single-cell RNA sequencing reveals SH3D21 promotes hepatocellular carcinoma progression by activating the PI3K/AKT/mTOR pathway

Authors - Wangxia Tong
  • KD Validated
View Article