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验证数据展示

  • WB analysis of human plasma using 85031-5-RR

    WB analysis of human plasma using 85031-5-RR

    human plasma was subjected to SDS PAGE followed by western blot with 85031-5-RR (C1QC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 85031-5-RR

    IHC staining of human cervical cancer using 85031-5-RR

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85031-5-RR (C1qC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C1QC antibody included
  • Affinity and Kinetic Characterization of 85031-5-RR

    Affinity and Kinetic Characterization of 85031-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85031-5-RR against Human C1qC were performed. The affinity constant is 1.26 nM.

C1qC Recombinant antibody

C1qC Recombinant Antibody for WB, IHC, ELISA
Cat No. 85031-5-RR

产品说明书

CloneNo. 242624B1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Complement C1q C chain, C1Q C, 242624B1, C1QG

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman plasma
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy C1QC Ready-To-Use IHC Kit. C1QC primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85031-5-RR targets C1qC in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 C1qC fusion protein Ag10400 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 complement component 1, q subcomponent, C chain
别名 Complement C1q C chain, C1Q C, 242624B1, C1QG
计算分子量 245 aa, 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC009016
基因名称 C1QC
Gene ID (NCBI) 714
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP02747
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qC which is the C-chain polypeptide of human complement subcomponent C1q.

实验方案

Product Specific Protocols
WB protocol for C1qC antibody 85031-5-RRDownload protocol
IHC protocol for C1qC antibody 85031-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols