验证数据展示
经过测试的应用
| Positive WB detected in | DU 145 cells, K-562 cells, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29362-1-AP targets C19orf72 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag30318 Product name: Recombinant human C19orf72 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 226-355 aa of BC013280 Sequence: RSSGSPEPSPAIAKAKEFVADIFRRAKEAKGGVPEEARPALCPGPSGSRCRAHSEPLALCGETAPRDSPPASEAPASEPGYVNYTKLYYVLESGEGTEPEDELEDDKISLPFVVTDLRGRNLRPMRERTA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 19 open reading frame 72 |
| 别名 | DCAF15 |
| 观测分子量 | 66 kDa |
| GenBank蛋白编号 | BC013280 |
| 基因名称 | C19orf72 |
| Gene ID (NCBI) | 90379 |
| RRID | AB_3669685 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q66K64 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C19orf72, also called DCAF15 (DDB1- and CUL4-associated factor 15), is the substrate-recognition component of the DCX complex, which is a cullin-4-RING E3 ubiquitin-protein ligase complex that mediates ubiquitination and degradation of target proteins (PMID:16949367; 31452512).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C19orf72 antibody 29362-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
