验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, mouse brain tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
产品信息
25514-1-AP targets C19orf70 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag22003 Product name: Recombinant human C19orf70 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-118 aa of BC042386 Sequence: MVARVWSLMRFLIKGSVAGVAVYLVYDQELLGPSDKSQAALQKAGEVVPPAMYQFSQYVCQQTGLQIPQLPAPPKIYFPIRDSWNAGIMTVMSALSVAPSKAREYSKEGWEYVKARTK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 19 open reading frame 70 |
| 别名 | MIC13, MICOS complex subunit MIC13, MICOS13, QIL1 |
| 计算分子量 | 118 aa, 13 kDa |
| 观测分子量 | 13 kDa |
| GenBank蛋白编号 | BC042386 |
| 基因名称 | C19orf70 |
| Gene ID (NCBI) | 125988 |
| RRID | AB_2880112 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5XKP0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C19orf70, also named as QIL1 and protein P117, belongs to the UPF0433 family. This antibody is specific to C19orf70.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for C19orf70 antibody 25514-1-AP | Download protocol |
| WB protocol for C19orf70 antibody 25514-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J Individual cristae within the same mitochondrion display different membrane potentials and are functionally independent. | ||
iScience Protease OMA1 modulates mitochondrial bioenergetics and ultrastructure through dynamic association with MICOS complex. | ||
Cytotherapy Stem cells from human exfoliated deciduous teeth affect mitochondria and reverse cognitive decline in a senescence-accelerated mouse prone 8 model. | ||
mBio Listeria monocytogenes Exploits Mitochondrial Contact Site and Cristae Organizing System Complex Subunit Mic10 To Promote Mitochondrial Fragmentation and Cellular Infection.
| ||
Cell Rep Mitochondrial GCN5L1 coordinates with YME1L and MICOS to remodel mitochondrial cristae in white adipocytes and modulate obesity |
