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验证数据展示

  • WB analysis using 27926-1-AP

    WB analysis using 27926-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27926-1-AP (C19orf62 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 27926-1-AP

    WB analysis of HepG2 using 27926-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27926-1-AP (C19orf62 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 27926-1-AP

    IHC staining of human breast cancer using 27926-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27926-1-AP (C19orf62 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 27926-1-AP

    IHC staining of human breast cancer using 27926-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27926-1-AP (C19orf62 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

C19orf62 Polyclonal antibody

C19orf62 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27926-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

BABAM1, C19orf62, HSPC142, MERIT40, NBA1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, U-251 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27926-1-AP targets C19orf62 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 C19orf62 fusion protein Ag27459 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 19 open reading frame 62
别名 BABAM1, C19orf62, HSPC142, MERIT40, NBA1
计算分子量 329 aa, 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC000788
基因名称 C19orf62
Gene ID (NCBI) 29086
RRIDAB_2881010
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NWV8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C19orf62 antibody 27926-1-APDownload protocol
IHC protocol for C19orf62 antibody 27926-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols