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验证数据展示

  • WB analysis of THP-1 using 86266-1-RR

    WB analysis of THP-1 using 86266-1-RR

    THP-1 cells were subjected to SDS PAGE followed by western blot with 86266-1-RR (C19orf59 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 86266-1-RR

    IF Staining of THP-1 using 86266-1-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using C19orf59 antibody (86266-1-RR, Clone: 250141G2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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C19orf59 Recombinant monoclonal antibody

C19orf59 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86266-1-RR

产品说明书

CloneNo. 250141G2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Mast cell-expressed membrane protein 1, MCEMP1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inTHP-1 cells
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86266-1-RR targets C19orf59 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag22616

Product name: Recombinant human C19orf59 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-187 aa of BC104798

Sequence: MEVEEIYKHQEVKMQAPAFRDKKQGVSAKNQGAHDPDYENITLAFKNQDHAKGGHSRPTSQVPAQCRPPSDSTQVPCWLYRAILSLYILLALAFVLCIILSAFIMVKNAEMSKELLGFKRELWNVSNSVQACEERQKRGWDSVQQSITMVRSKIDRLETTLAGIKNIDTKVQKILEVLQKMPQSSPQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 19 open reading frame 59
别名 Mast cell-expressed membrane protein 1, MCEMP1
观测分子量22/24 kDa
GenBank蛋白编号BC104798
基因名称 MCEMP1
Gene ID (NCBI) 199675
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8IX19
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The MCEMP1 gene contains seven exons and was mapped to human chromosome 19p13.3. The epitope-tagged MCEMP1 has been expressed in mammalian cells and found to be localized to the cellular membrane with its C-terminus extending to the outside of the membrane and N-terminus into the cytoplasmic compartment. An evidently increased expression of mast cell expression membrane protein 1 (MCEMP1) has been observed in stroke patients and its expression independently improved the discrimination of 1-month outcome, suggesting the functionality of peripheral blood expression of MCEMP1 as a biomarker for stroke prognosis . (PMID: 30883005, PMID: 31104315, PMID: 15953541)

实验方案

Product Specific Protocols
WB protocol for C19orf59 antibody 86266-1-RRDownload protocol
IF protocol for C19orf59 antibody 86266-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols