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验证数据展示

  • WB analysis using 25050-1-AP

    WB analysis using 25050-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25050-1-AP (C19orf57 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 25050-1-AP

    IHC staining of mouse testis using 25050-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25050-1-AP (C19orf57 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Jurkat using 25050-1-AP

    WB analysis of Jurkat using 25050-1-AP

    WB result of C19orf57 antibody (25050-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C19orf57 transfected Jurkat cells.

  • WB analysis of PC-3 using 25050-1-AP

    WB analysis of PC-3 using 25050-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25050-1-AP (C19orf57 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 25050-1-AP

    IF Staining of A431 using 25050-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C19orf57 antibody (25050-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Featured Product
  • KD/KO验证

C19orf57 Polyclonal antibody

C19orf57 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25050-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Pre-T/NK cell-associated protein 3B3, BRME1, Break repair meiotic recombinase recruitment factor 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, PC-3 cells, A431 cells, A2780 cells, MOLT-4 cells, mouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25050-1-AP targets C19orf57 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag21745

Product name: Recombinant human C19orf57 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 281-637 aa of BC119719

Sequence: ASAPTSGPAPGLGPASWCLEPGSVAQGSPDPQQTPSRMGREGEGTHSSLGCSSLGMVVIADLSTDPTELEERALEVAGPDGQASAISPASPRRKAADGGHRRALPGCTSLTGETTGESGEAGQDGKPPGDVLVGPTASLALAPGSGESMMGAGDSGHASPDTGPCVNQKQEPGPAQEEAELGGQNLERDLEGFRVSPQASVVLEHREIADEPLQEPGAQQGIPDTTSELAGQRDHLPHSADQGTWADSLAVELDFLLDSQIQDALDASDFEAPPEQLFPSGNKPGPCWPGPSSHANGDPVAVAKAQPSRLIMGTHRDLEAFKRLNYRKTKLGGKAPLPYPSKGPGNIPRGDPPWREL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 19 open reading frame 57
别名 Pre-T/NK cell-associated protein 3B3, BRME1, Break repair meiotic recombinase recruitment factor 1
计算分子量 668 aa, 70 kDa
观测分子量 60-100 kDa
GenBank蛋白编号BC119719
基因名称 C19orf57
Gene ID (NCBI) 79173
RRIDAB_2879871
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ0VDD7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C19orf57 antibody 25050-1-APDownload protocol
IHC protocol for C19orf57 antibody 25050-1-APDownload protocol
IF protocol for C19orf57 antibody 25050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols