验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HEK-293 cells |
| Positive IHC detected in | human colon tissue, human gliomas tissue, human stomach cancer tissue, mouse colon tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19420-1-AP targets C19orf43 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag13747 Product name: Recombinant human C19orf43 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-176 aa of BC000216 Sequence: MAARGRRAEPQGREAPGPAGGGGGGSRWAESGSGTSPESGDEEVSGAGSSPVSGGVNLFANDGSFLELFKRKMEEEQRQRQEEPPPGPQRPDQSAAAAGPGDPKRKGGPGSTLSFVGKRRGGNKLALKTGIVAKKQKTEDEVLTSKGDAWAKYMAEVKKYKAHQCGDDDKTRPLVK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 19 open reading frame 43 |
| 别名 | TRIR, EC:3.1.13.-, Exoribonuclease TRIR, fSAP18, Telomerase RNA component interacting RNase |
| 计算分子量 | 176 aa, 18 kDa |
| 观测分子量 | 28 kDa |
| GenBank蛋白编号 | BC000216 |
| 基因名称 | C19orf43 |
| Gene ID (NCBI) | 79002 |
| RRID | AB_10640586 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BQ61 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C19orf43, also named as MGC2803 and fSAP18, is an Uncharacterized protein. It is 176aa,18.4kd. We used the antibody raised from the C19orf43 protein, we get a ~28kd in human placenta, 293 and A431 cell.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for C19orf43 antibody 19420-1-AP | Download protocol |
| WB protocol for C19orf43 antibody 19420-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |






