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验证数据展示

  • WB analysis of mouse testis using 25650-1-AP

    WB analysis of mouse testis using 25650-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25650-1-AP (C19orf36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 25650-1-AP

    IHC staining of mouse testis using 25650-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25650-1-AP (C19orf36 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of mouse testis using 25650-1-AP

    IHC staining of mouse testis using 25650-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25650-1-AP (C19orf36 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 25650-1-AP

    IF Staining of HepG2 using 25650-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C19orf36 antibody (25650-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

C19orf36 Polyclonal antibody

C19orf36 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25650-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

IZUMO4, Izumo sperm-egg fusion protein 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, HepG2 cells
Positive IHC detected inmouse testis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25650-1-AP targets C19orf36 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性bovine
免疫原 C19orf36 fusion protein Ag20434 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 19 open reading frame 36
别名 IZUMO4, Izumo sperm-egg fusion protein 4
计算分子量 232 aa, 27 kDa
观测分子量 27-30 kDa
GenBank蛋白编号BC014609
基因名称 C19orf36
Gene ID (NCBI) 113177
RRIDAB_2880175
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ1ZYL8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IZUMO4, also named as C19orf36, is sperm 22 kDa protein c113. It has 4 isoforms with MW 17-27 kDa.

实验方案

Product Specific Protocols
WB protocol for C19orf36 antibody 25650-1-APDownload protocol
IHC protocol for C19orf36 antibody 25650-1-APDownload protocol
IF protocol for C19orf36 antibody 25650-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
bovineIF,WB

Int J Biol Macromol

TMT-based quantitative N-glycoproteomic analysis reveals glycoprotein protection can improve the quality of frozen bovine sperm.

Authors - Na Wang
View Article