验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, HepG2 cells |
| Positive IHC detected in | mouse testis tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
25650-1-AP targets C19orf36 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | bovine |
| 免疫原 |
CatNo: Ag20434 Product name: Recombinant human C19orf36 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 16-214 aa of BC014609 Sequence: HGCLHCHSNFSKKFSFYRHHVNFKSWWVGDIPVSGALLTDWSDDTMKELHLAIPAKITREKLDQVATAVYQMMDQLYQGKMYFPGYFPNELRNIFREQVHLIQNAIIESRIDCQHRCGIFQYETISCNNCTDSHVACFGYNCESSAQWKSAVQGLLNYINNWHKQDTSMSLVSPALRCLEPPHLANLTLEDAAECLKQH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 19 open reading frame 36 |
| 别名 | Izumo sperm-egg fusion protein 4, IZUMO4 |
| 计算分子量 | 232 aa, 27 kDa |
| 观测分子量 | 27-30 kDa |
| GenBank蛋白编号 | BC014609 |
| 基因名称 | C19orf36 |
| Gene ID (NCBI) | 113177 |
| RRID | AB_2880175 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q1ZYL8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IZUMO4, also named as C19orf36, is sperm 22 kDa protein c113. It has 4 isoforms with MW 17-27 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C19orf36 antibody 25650-1-AP | Download protocol |
| IHC protocol for C19orf36 antibody 25650-1-AP | Download protocol |
| IF protocol for C19orf36 antibody 25650-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
