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验证数据展示

  • WB analysis of COLO 320 using 24977-1-AP

    WB analysis of COLO 320 using 24977-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 24977-1-AP (C18orf21 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 24977-1-AP

    IHC staining of human pancreas using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with C18orf21 antibody included
  • IF Staining of COLO 320 using 24977-1-AP

    IF Staining of COLO 320 using 24977-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed COLO 320 cells using 24977-1-AP (C18orf21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human thyroid cancer using 24977-1-AP

    IHC staining of human thyroid cancer using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C18orf21 antibody included
  • IHC staining of human thyroid cancer using 24977-1-AP

    IHC staining of human thyroid cancer using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C18orf21 antibody included
  • IHC staining of human urothelial carcinoma using 24977-1-AP

    IHC staining of human urothelial carcinoma using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C18orf21 antibody included
  • IHC staining of human urothelial carcinoma using 24977-1-AP

    IHC staining of human urothelial carcinoma using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C18orf21 antibody included

C18orf21 Polyclonal antibody

C18orf21 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24977-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

XTP13, PNAS-131, PNAS-124, HsT3108, HBV X-transactivated gene 13 protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman urothelial carcinoma tissue, human colon cancer tissue, human pancreas tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCOLO 320 cells
Planning an IHC experiment? We recommend our IHCeasy C18orf21 Ready-To-Use IHC Kit. C18orf21 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24977-1-AP targets C18orf21 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag21782

Product name: Recombinant human C18orf21 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-220 aa of BC119766

Sequence: MRQKHYLEAAARGLHDSCPGQARYLLWAYTSSHDDKSTFEETCPYCFQLLVLDNSRVRLKPKARLTPKIQKLLNREARNYTLSFKEAKMVKKFKDSKSVLLITCKTCNRTVKHHGKSRSFVSTLKSNPATPTSKLSLKTPERRTANPNHDMSGSKGKSPASVFRTPTSGQSVSTCSSKNTSKTKKHFSQLKMLLSQNESQKIPKVDFRNFLSSLKGGLLK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 18 open reading frame 21
别名 XTP13, PNAS-131, PNAS-124, HsT3108, HBV X-transactivated gene 13 protein
计算分子量 220 aa, 25 kDa
观测分子量 25-28 kDa
GenBank蛋白编号BC119766
基因名称 C18orf21
Gene ID (NCBI) 83608
RRIDAB_2879829
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ32NC0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C18orf21, also named as XTP13, belongs to the UPF0711 family. The MW is 25-28 kDa in WB detection.

实验方案

Product Specific Protocols
IF protocol for C18orf21 antibody 24977-1-APDownload protocol
IHC protocol for C18orf21 antibody 24977-1-APDownload protocol
WB protocol for C18orf21 antibody 24977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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