×
Use Able AI chat for product recommendations

验证数据展示

  • IHC staining of human pancreas using 24977-1-AP

    IHC staining of human pancreas using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with C18orf21 antibody included
  • IF Staining of COLO 320 using 24977-1-AP

    IF Staining of COLO 320 using 24977-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed COLO 320 cells using 24977-1-AP (C18orf21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 24977-1-AP

    WB analysis using 24977-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24977-1-AP (C18orf21 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid cancer using 24977-1-AP

    IHC staining of human thyroid cancer using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C18orf21 antibody included
  • IHC staining of human thyroid cancer using 24977-1-AP

    IHC staining of human thyroid cancer using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C18orf21 antibody included
  • IHC staining of human urothelial carcinoma using 24977-1-AP

    IHC staining of human urothelial carcinoma using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C18orf21 antibody included
  • IHC staining of human urothelial carcinoma using 24977-1-AP

    IHC staining of human urothelial carcinoma using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C18orf21 antibody included

C18orf21 Polyclonal antibody

C18orf21 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24977-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

HBV XAg-transactivated protein 13, HBV X-transactivated gene 13 protein, HsT3108, PNAS-124, PNAS-131

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inCOLO 320 cells, Jurkat cells
Positive IHC detected inhuman urothelial carcinoma tissue, human colon cancer tissue, human pancreas tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCOLO 320 cells
Planning an IHC experiment? We recommend our IHCeasy C18orf21 Ready-To-Use IHC Kit. C18orf21 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24977-1-AP targets C18orf21 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag21782

Product name: Recombinant human C18orf21 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-220 aa of BC119766

Sequence: MRQKHYLEAAARGLHDSCPGQARYLLWAYTSSHDDKSTFEETCPYCFQLLVLDNSRVRLKPKARLTPKIQKLLNREARNYTLSFKEAKMVKKFKDSKSVLLITCKTCNRTVKHHGKSRSFVSTLKSNPATPTSKLSLKTPERRTANPNHDMSGSKGKSPASVFRTPTSGQSVSTCSSKNTSKTKKHFSQLKMLLSQNESQKIPKVDFRNFLSSLKGGLLK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 18 open reading frame 21
别名 HBV XAg-transactivated protein 13, HBV X-transactivated gene 13 protein, HsT3108, PNAS-124, PNAS-131
计算分子量 220 aa, 25 kDa
观测分子量 25-28 kDa
GenBank蛋白编号BC119766
基因名称 C18orf21
Gene ID (NCBI) 83608
RRIDAB_2879829
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ32NC0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C18orf21, also named as XTP13, belongs to the UPF0711 family. The MW is 25-28 kDa in WB detection.

实验方案

Product Specific Protocols
IF protocol for C18orf21 antibody 24977-1-APDownload protocol
IHC protocol for C18orf21 antibody 24977-1-APDownload protocol
WB protocol for C18orf21 antibody 24977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nat Struct Mol Biol

RNase MRP subunit composition and role in 40S ribosome biogenesis.

Authors - Eric M. Smith
  • KO Validated
View Article
||
New chat

Able™

正在加载，请稍候...