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C18orf21 Polyclonal antibody

C18orf21 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 24977-1-AP

Print datasheet

Synonyms

C18orf21, HsT3108, PNAS 124, PNAS 131, UPF0711 protein C18orf21, XTP13

验证数据展示

  • WB analysis of COLO 320 using 24977-1-AP

    WB analysis of COLO 320 using 24977-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 24977-1-AP (C18orf21 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 24977-1-AP

    IHC staining of human colon cancer using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with C18orf21 antibody included
  • IHC staining of human pancreas using 24977-1-AP

    IHC staining of human pancreas using 24977-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with C18orf21 antibody included
  • IF Staining of COLO 320 using 24977-1-AP

    IF Staining of COLO 320 using 24977-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed COLO 320 cells using 24977-1-AP (C18orf21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman pancreas tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCOLO 320 cells
Planning an IHC experiment? We recommend our IHCeasy C18orf21 Ready-To-Use IHC Kit. C18orf21 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24977-1-AP is C18orf21 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human
Immunogen C18orf21 fusion protein Ag21782 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 18 open reading frame 21
Synonyms C18orf21, HsT3108, PNAS 124, PNAS 131, UPF0711 protein C18orf21, XTP13
Calculated Molecular Weight 220 aa, 25 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC119766
Gene Symbol C18orf21
Gene ID (NCBI) 83608
RRIDAB_2879829
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ32NC0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C18orf21, also named as XTP13, belongs to the UPF0711 family. The MW is 25-28 kDa in WB detection.

实验方案

Product Specific Protocols
WB protocol for C18orf21 antibody 24977-1-APDownload protocol
IHC protocol for C18orf21 antibody 24977-1-APDownload protocol
IF protocol for C18orf21 antibody 24977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols