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验证数据展示

  • WB analysis of Jurkat using 27114-1-AP

    WB analysis of Jurkat using 27114-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 27114-1-AP (C17orf49 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-3 using 27114-1-AP

    IP experiment of PC-3 using 27114-1-AP

    IP result of anti-C17orf49 (IP:27114-1-AP, 4ug; Detection:27114-1-AP 1:1500) with PC-3 cells lysate 3840 ug.

  • IHC staining of human colon using 27114-1-AP

    IHC staining of human colon using 27114-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27114-1-AP (C17orf49 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C17orf49 antibody included
  • IHC staining of human colon using 27114-1-AP

    IHC staining of human colon using 27114-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27114-1-AP (C17orf49 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C17orf49 antibody included

C17orf49 Polyclonal antibody

C17orf49 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 27114-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

C17orf49

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, PC-3 cells, mouse ovary tissue
Positive IP detected inPC-3 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy C17orf49 Ready-To-Use IHC Kit. C17orf49 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27114-1-AP targets C17orf49 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag25882

Product name: Recombinant human C17orf49 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-172 aa of BC040036

Sequence: MTSASTKVGEIFSAAGAAFTKLGELTMQLHPVADSSPAGAKWTETEIEMLRAAVKRFGDDLNHISCVIKERTVAQIKATVKRKVYEDSGIPLPAESPKKGPKKVASGVLSPPPAAPPPSSSSVPEAGGPPIKKQKADVTLSALNDSDANSDVVDIEGLGETPPAKKLNFDQA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 17 open reading frame 49
别名 C17orf49
观测分子量 22-25 kDa
GenBank蛋白编号BC040036
基因名称 C17orf49
Gene ID (NCBI) 124944
RRIDAB_2880762
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IXM2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C17orf49 antibody 27114-1-APDownload protocol
IHC protocol for C17orf49 antibody 27114-1-APDownload protocol
IP protocol for C17orf49 antibody 27114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols