• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

C17orf49 Polyclonal antibody

C17orf49 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 27114-1-AP

Print datasheet

Synonyms

C17orf49

验证数据展示

  • WB analysis of Jurkat using 27114-1-AP

    WB analysis of Jurkat using 27114-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 27114-1-AP (C17orf49 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-3 using 27114-1-AP

    IP experiment of PC-3 using 27114-1-AP

    IP result of anti-C17orf49 (IP:27114-1-AP, 4ug; Detection:27114-1-AP 1:1500) with PC-3 cells lysate 3840 ug.

  • IHC staining of human colon using 27114-1-AP

    IHC staining of human colon using 27114-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27114-1-AP (C17orf49 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C17orf49 antibody included
  • IHC staining of human colon using 27114-1-AP

    IHC staining of human colon using 27114-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27114-1-AP (C17orf49 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C17orf49 antibody included

经过测试的应用

Positive WB detected inJurkat cells, PC-3 cells, mouse ovary tissue
Positive IP detected inPC-3 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy C17orf49 Ready-To-Use IHC Kit. C17orf49 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27114-1-AP targets C17orf49 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IP, WB, ELISA
Tested Reactivity human, mouse
Immunogen C17orf49 fusion protein Ag25882 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 17 open reading frame 49
Synonyms C17orf49
Observed Molecular Weight 22-25 kDa
GenBank Accession NumberBC040036
Gene Symbol C17orf49
Gene ID (NCBI) 124944
RRIDAB_2880762
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IXM2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C17orf49 antibody 27114-1-APDownload protocol
IHC protocol for C17orf49 antibody 27114-1-APDownload protocol
IP protocol for C17orf49 antibody 27114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols