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验证数据展示

  • WB analysis of Raji using 25364-1-AP

    WB analysis of Raji using 25364-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 25364-1-AP (C16orf93 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 25364-1-AP

    IF Staining of HEK-293 using 25364-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using C16orf93 antibody (25364-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 25364-1-AP

    IF Staining of HeLa using 25364-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using C16orf93 antibody (25364-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

C16orf93 Polyclonal antibody

C16orf93 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25364-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

BTBD12, CCDC189, CFAP119, Cilia- and flagella-associated protein 119, Coiled-coil domain-containing protein 189

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRaji cells
Positive IF/ICC detected inHeLa cells, HEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25364-1-AP targets C16orf93 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 C16orf93 fusion protein Ag21676 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 16 open reading frame 93
别名 BTBD12, CCDC189, CFAP119, Cilia- and flagella-associated protein 119, Coiled-coil domain-containing protein 189
计算分子量 331 aa, 38 kDa
观测分子量 34 kDa
GenBank蛋白编号BC128129
基因名称 C16orf93
Gene ID (NCBI) 90835
RRIDAB_2880045
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA1A4V9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C16orf93, also named as CCDC189, is a coiled-coil domain-containing protein which has four isoforms with MW 38 kDa, 34 kDa, 12 kDa and 20 kDa.

实验方案

Product Specific Protocols
WB protocol for C16orf93 antibody 25364-1-APDownload protocol
IF protocol for C16orf93 antibody 25364-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols