验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, SW 1990 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
30246-1-AP targets C16orf74 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag32878 Product name: Recombinant human C16orf74 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 10-76 aa of BC009078 Sequence: GFQMCVSSSSSSHDEAPVLNDKHLDVPDIIITPPTPTGMMLPRDLGSTVWLDETGSCPDDGEIDPEA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 16 open reading frame 74 |
| 别名 | Uncharacterized protein C16orf74 |
| 观测分子量 | 15 - 20 kDa |
| GenBank蛋白编号 | BC009078 |
| 基因名称 | C16orf74 |
| Gene ID (NCBI) | 404550 |
| RRID | AB_3086278 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96GX8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The C16orf74 has been reported associated with tumor necrosis factor (TNF)-alpha as well as hypoxic condition. Moreover, several reports have indicated that C16orf74 expression is a potential prognostic factor in several types of cancer. The results of several genome-wide studies have indicated that C16orf74 is involved in inflammatory processes. C16orf74 is overexpressed in the vast majority of human PDAC(Clinical outcome of pancreatic ductal adenocarcinoma), and likely plays a significant role in pancreatic cell growth and invasion through its interaction with PPP3CA.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for C16orf74 antibody 30246-1-AP | Download protocol |
| WB protocol for C16orf74 antibody 30246-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
