验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, MOLT-4 cells, Raji cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26923-1-AP targets VPS35L in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | VPS35L fusion protein Ag25620 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 16 open reading frame 62 |
| 别名 | UPF0505 protein C16orf62, RTSC3, Esophageal cancer-associated protein, EC97, C16orf62 |
| 观测分子量 | 98 kDa |
| GenBank蛋白编号 | BC058845 |
| 基因名称 | C16orf62 |
| Gene ID (NCBI) | 57020 |
| RRID | AB_3669574 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z3J2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VPS35L, also known as EC97, contains 5 N-glycosylation sites, 3 N-myristoylation sites, and numerous phosphorylation sites. Northern blot analysis detected a 3.4-kb EC97 transcript in all human tissues tested except adipose. RT-PCR analysis showed that EC97 expression was upregulated in 60% of human esophageal squamous cell carcinoma (ESCC) tissues and downregulated in 35% of ESCC tissues tested compared with controls. VPS35L is Involved in Golgi to plasma membrane transport and endocytic recycling.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VPS35L antibody 26923-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |